Abstract 12774: Effects of Interleukin-1β on Cardiac Reserve and Exercise Capacity in the Mouse

• Published in: Circulation (New York, N.Y.) N.Y.), 2021-11, Vol.144 (Suppl_1), p.A12774-A12774
• Main Authors: Vohra, Habeebah Z, MEZZAROMA, Eleonora, Abbate, Antonio, Toldo, Stefano
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.144.suppl_1.12774

IntroductionHeart failure (HF) is characterized by dyspnea, fatigue, and exercise intolerance. Clinical evidence points to increased interleukin-1β (IL-1β) activity in patients with HF, with IL-1 blockade improving the exercise capacity in HF patients. In healthy mice, recombinant-mouse IL-1β (rmIL-1β) induces acute systolic dysfunction, peaking 4 hours after administration. However, the direct effects of rmIL-1β on exercise capacity are unknown. HypothesisrmIL-1β diminishes the exercise capacity in the mouse. MethodsAdult mice were trained to run on a treadmill and exercise capacity was assessed before, 4, and 96 hours after intraperitoneal rmIL-1β (3 μg/kg) or vehicle (0.9% NaCl) administration (N=7-10/group). In separate groups of mice, left ventricular ejection fraction (LVEF) was assessed at baseline, 4, and 96 hours after stimulation using transthoracic echocardiography. The ultrasound operator was blind to treatments. The cardiac reserve was measured by calculating the difference in LVEF before and after β-adrenergic stimulation using isoproterenol (10 ng/mouse) in IL-1β or vehicle-treated mice (N=5-11/group). ResultsTreatment with rmIL-1β significantly reduced exercise capacity measured at 4 and 96 hours (32% reduction at 4 hours and 33% reduction at 96 hours, P