Abstract 14076: Freeze-Dried Human Placental Mesenchymal Stem Cell Prevents the Progression of Acute Respiratory Distress Syndrome in Rats

• Published in: Circulation (New York, N.Y.) N.Y.), 2021-11, Vol.144 (Suppl_1), p.A14076-A14076
• Main Authors: Kipshidze, Nodar, Kakabadze, Zurab, Paresishvili, Tea, Chakhunashvili, David, Kipshidze, Nicholas
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.144.suppl_1.14076

IntroductionAcute respiratory distress syndrome (ARDS) is one of the main causes of mortality in patients with COVID-19. Despite numerous studies, therapies for ARDS in COVID-19 remain significantly limited. Currently, there have been reports of the efficient use of mesenchymal stem cell (MSC) for the treatment of ARDS. Several studies have reported that MSC produces anti-inflammatory cytokines, promotes the maturation of dendritic cells, and modulates NK cells. The safety of MSC therapy has been demonstrated in early-stage clinical studies with a relatively small number of patients. We investigated the influence of freeze-dried human placental mesenchymal stem cells (HPMSC) in a preclinical rodent model of ARDS. MethodsSixty rats were randomly divided into three groups of 20the first group was the control, the second group received dexamethasone, and the third group received HPMSC. ResultsSurvival was highest in the HPMSC group (75%, 95%CI50, 88) when compared to the control group. In a cox regression model, mortality of HPMSC group (HR11.30 [95% CI4.05, 31.50]) when compared to control was statistically significant (p < 0.001). Mortality in the dexamethasone (HR64.25 [95% CI32.71, 126.19]) was not statistically significant (p = 0.199). Plasma serum TNF-α levels were statistically significant in the HMPSC group when compared to the control (p < 0.001) and dexamethasone group (p < 0.001). Single factor ANOVA comparing TNF-α levels between all groups was also significant (p < 0.0001). Trends (Table 1) indicated that plasma serum cytokine levels for TNF-α, IL-1β, and Il-6 were less in the HPMSC group than the control and dexamethasone groups. ConclusionsThese experiments demonstrated that HPMSC significantly decreased mortality, endogenous intoxication, and a reduction in the release of pro-inflammatory cytokines. The freeze-dried placental stem cell is a promising biomaterial that can be used nebulized for the treatment of coronavirus disease.