Abstract 364: Metabolic Syndrome Induced by High Fat/high Fructose Diet in C57bl/6 Mice Impaired Cardiac Remodeling During Pregnancy

• Published in: Circulation research 2018-08, Vol.123 (Suppl_1)
• Main Authors: Yang, Yijun, Joo, Yunichel, Wang, Tao, Borghetti, Giulia, Harper, Shavonn, Bryan, Christopher, Mohsin, Sadia, Houser, Steven R
• Format: Article
• Language: English
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/res.123.suppl_1.364

Abstract only Rationale: Due to the change of life style in the past decades, the incidence of metabolic syndrome (MetS) and obesity in women of childbearing age has been increased. Evidences have shown that complications during pregnancy, such as obesity and gestational diabetes, strongly link to future heart diseases. During pregnancy, the heart undergoes a physiological remodeling with mild ventricular dilation and hypertrophy. However, the effects of MetS on cardiac phenotype during pregnancy are not well understood. Moreover, animal models for MetS so far are not well established, which are usually species limited, gender limited and lack of translational significance. Thus, it’s important to develop an animal model which resembles the etiology of MetS in human beings. Objective: Establish a model of MetS in female C57BL/6J mice and determine the effects of MetS on cardiac remodeling during pregnancy. Methods and Results: To establish a mouse model of MetS, high fat (49% kcal)/high fructose (35.6% kcal) diet are given to C57BL/6J mice at the age of 8-week. Mice fed with normal diet (10% kcal fat) served as control. To better characterize the model within different genders, male mice are also subjected to the high fat/ high fructose (HFHF diet) and control diet. After 12 weeks feeding, mice fed with HFHF diet showed significantly higher body weight and gonadal fat weight compared with control mice in both genders. Also, mice fed with HFHF diet showed impaired glucose tolerance and higher serum total cholesterol level. However, the blood pressure showed no difference between groups in both genders. Overall, the symptoms of obesity, impaired glucose tolerance and dyslipidemia suggest the development of MetS. Female mice after 12-week special diet feeding will be subjected to breeding. Cardiac function is then evaluated by echocardiography before and during pregnancy. Female mice that developed MetS showed impaired cardiac function and pathological remodeling during pregnancy. Conclusion: We’ve developed a mouse model of MetS in both female and male C57BL/6J mice strain with 12 weeks feeding of HFHF diet, which resemble the western diet that affects human beings nowadays. Females that have MetS showed impaired cardiac function and pathological remodeling during pregnancy.