Abstract 227: Intravital Multiphoton Microscopy Reveals Increased Capillary Patrolling by Leukocytes and Cardiomyocyte Dysfunction in High Fat Diet Induced Hypertrophy

• Published in: Circulation research 2019-08, Vol.125 (Suppl_1)
• Main Authors: Small, David M, Allan-Rahill, Nathaniel H, Lamont, Michael R, Djakpa, Salomon, Jhala, Marvarakumari G, Zhu, Yvette, Nishimura, Nozomi
• Format: Article
• Language: English
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/res.125.suppl_1.227

Abstract only Background: The study of functional cardiomyocyte adaptation and inflammatory cell behavior at the micro-scale in vivo has been challenging due to limited imaging tools. We recently developed intravital multiphoton microscopy (MPM) methods that enable visualization and quantification of cardiac dynamics at a cell-scale throughout the cardiac cycle. We aimed to determine the dynamic cellular changes that occur due to high fat diet (HFD) induced hypertrophy using intravital cardiac MPM. Methods: ApoE -/- C57Bl6 mice started a HFD at 6 weeks of age (ApoE -/- -HFD, n=11), while age-matched wild-type mice (WT-ND, n=10) were fed a normal chow diet. At 26-weeks, mice were assessed by cardiac echocardiography and intravital MPM in the intact beating heart. Intravenous injections of rhodamine-6G (R6g) labeled cardiomyocytes and leukocytes, and Texas-Red dextran labeled vasculature. 3D volumes were reconstructed throughout the cardiac cycle to quantify cell motion using automated algorithms. Results: ApoE -/- -HFD hearts underwent hypertrophy compared to WT-ND with increased heart weight-to-tibial length ratio (10±0.8 vs 13±1.1) and left ventricle wall thickness (1.07±0.03 mm vs 1.13±0.06 mm, respectively, p