The Toxicological Properties of Petroleum Gases

• Published in: International journal of toxicology 2014-01, Vol.33 (1_suppl), p.28S-51S
• Main Authors: McKee, Richard H., Herron, Deborah, Saperstein, Mark, Podhasky, Paula, Hoffman, Gary M., Roberts, Linda
• Format: Article
• Language: English
• Subjects: Administration, Inhalation; Alkanes - analysis; Alkanes - toxicity; Animals; Gases - analysis; Gases - toxicity; Hazardous Substances - analysis; Hazardous Substances - toxicity; Micronucleus Tests; No-Observed-Adverse-Effect Level; Petroleum - analysis; Petroleum - toxicity; Prenatal Diagnosis; Rats; Rats, Sprague-Dawley; Reproduction - drug effects; Toxicity Tests
• ISSN: 1091-5818; 1092-874X
• DOI: 10.1177/1091581813504225

To characterize the toxicological hazards of petroleum gases, 90-day inhalation toxicity (Organization for Economic Cooperation and Development [OECD] 413) and developmental toxicity (OECD 414) tests were conducted with liquefied propane gas (LPG) at concentrations of 1000, 5000, or 10 000 ppm. A micronucleus test (OECD 474) of LPG was also conducted. No systemic or developmental effects were observed; the overall no observed adverse effect concentration (NOAEC) was 10 000 ppm. Further, there was no effect of LPG exposure at levels up to 10 000 ppm on micronucleus induction and no evidence of bone marrow toxicity. Other alkane gases (ethane, propane, n-butane, and isobutane) were then evaluated in combined repeated exposure studies with reproduction/development toxicity screening tests (OECD 422). There were no toxicologically important changes in parameters relating to systemic toxicity or neurotoxicity for any of these gases at concentrations ranging from 9000 to 16 000 ppm. There was no evidence of effects on developmental or reproductive toxicity in the studies of ethane, propane, or n-butane at the highest concentrations tested. However, there was a reduction in mating in the high-exposure group (9000 ppm) of the isobutane study, which although not significantly different was outside the range previously observed in the testing laboratory. Assuming the reduction in mating to have been toxicologically significant, the NOAEC for the isobutane reproductive toxicity screening test was 3000 ppm (7125 mg/m3). A method is proposed by which the toxicity of any of the 106 complex petroleum gas streams can be estimated from its composition.