The demonstration, staining and prevalences, in pathological and non-pathological specimens, of eosinophils in faeces

Although eosinophils are occasionally reported in mucus there has been no description of them in faecal samples. Attempts were made to demonstrate eosinophils in stool samples using several different staining techniques. Use of an acid dye, Acid Red 29, was found to be the simplest and most direct m...

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Bibliographic Details
Published in:Annals of tropical medicine and parasitology 2002-07, Vol.96 (5), p.523-528
Main Authors: Petithory, J.-C., Ardoin, F. G.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Eosinophils - cytology
Eosinophils - pathology
Feces - cytology
Helminthic diseases
Humans
Infectious diseases
Intestinal Diseases - pathology
Intestinal Diseases, Parasitic - pathology
Medical sciences
Miscellaneous
Parasitic diseases
Rhodamines
Staining and Labeling - methods
Tissue Preservation
Tropical medicine
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Summary:Although eosinophils are occasionally reported in mucus there has been no description of them in faecal samples. Attempts were made to demonstrate eosinophils in stool samples using several different staining techniques. Use of an acid dye, Acid Red 29, was found to be the simplest and most direct method of revealing eosinophils, producing very characteristic, orange-brown, sometimes red, staining of the intracellular granules that contain eosinophil cationic protein. In stool samples held at room temperature, without preservative, eosinophils remained demonstrable for a mean of 15 days and occasionally for a year. The peroxidase in the eosinophilic granules may help to preserve the cells. Eosinophils were found in 32 (14%) of 223 stool samples from patients with intestinal disease (including the results of several parasitic infections) but in none of 72 samples from apparently healthy hospital personnel (P
ISSN:0003-4983
1364-8594
DOI:10.1179/000349802125001302