Study on the Subtypes of Dendritic Cells and the Expression of T Cell Co-Stimulating Molecules (CD80, CD86, CD40) on Dendritic Cells and B Cells in the Peripheral Blood of SAA Patients

• Published in: Blood 2006-11, Vol.108 (11), p.3753-3753
• Main Authors: Shao, Zonghong, Tu, Meifeng, Liu, Hong, He, Guangsheng, Shi, Jun, Bai, Jie, Cao, Yanran, Wang, Huaquan, Xin, Limin, Cui, Zhenzhu, Sun, Juan, Jia, Hairong, Chen, Huisu, Xue, Yanping, Yang, Chongli
• Format: Article
• Language: English
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V108.11.3753.3753

Objective To detect the quantities of monocyte-derived dendritic cell precursors (pDC1) and plasmacytoid dendritic cell precursors (pDC2) in peripheral blood mononuclear cells (PBMC) of severe aplastic anemia (SAA) patients before and after immune suppressive therapy (IST), the ratio of their pDC1 to pDC2, and the expression of T-cell co-stimulating molecules (CD80, CD86, CD40) on dentritic cells (DC) and B cells surface in the SAA patients' peripheral blood. Methods with three-color monoclonal antibody labeling technology, the quantities and ratio of pDC1 and pDC2 in PBMC were detected in 26 patients with SAA at active phase,13 patients with SAA at recovery phase and 15 normal control respectively by FACS. The aforementioned merits of 10 SAA patients were tested before and 2 months after IST by FACS. By FACS, the expression of CD80, CD86 and CD40 on DC and B lymphocytes were detected in 16 patients with SAA and 15 normal controls. Results The percentages of total pDC, pDC1, pDC2 and the ratio of pDC1/pDC2 of controls (healthy people) were(0.72±0.32)%,(0.41±0.18)%,(0.30±0.21)%, 1.58±0.69 respectively, and those of the patients with SAA at active phase were(0.96±0.92)%,(0.67±0.65)%,(0.32±0.30)%,2.70±1.63 respectively. The differences were significant [pDC1 (P