Epicatechin Impact on Primary Hemostasis, Coagulation and Fibrinolysis

• Published in: Blood 2015-12, Vol.126 (23), p.4677-4677
• Main Authors: Sinegre, Thomas, Mazur, André, Berger, Marc G, Milenkovic, Dragan, Sapin, Anne-Françoise, Morand, Christine, Lebreton, Aurelien
• Format: Article
• Language: English
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V126.23.4677.4677

Introduction Cardiovascular diseases are the leading causes of death worldwide. Among cardiovascular risk factors, some are linked to the stages of hemostasis : primary haemostasis, coagulation and fibrinolysis. There is a growing interest on the potential beneficial effects of some nutritional components on cardiovascular health, in particular in the field of thrombosis. Dark chocolate is associated with a one-third reduction in the risk of cardiovascular disease. Cocoa is particularly rich in a flavonoid subclass called flavan-3-ol which includes epicatechin. Epicatechin is a micronutrient found mainly in chocolate, green tea and grape. It has already been proven that epicatechin impacts platelet functions but there are only poor data on the role of epicatechin on coagulation and fibrinolysis. The aim of this study was to evaluate the impact of epicatechin in vitro on the different stages of hemostasis. Patients and methods The study was performed on 10 major healthy subjects. Exclusion criteria were antiplatelet or anticoagulant therapy intake, clinical history of thrombosis or bleeding, thrombocytopenia (platelet count < 150 G/L) and abnormal coagulation profile (fibrinogen < 1.8 g/L, Prothrombin time > 13 s, and activated partial thromboplastin time > 39 s). Platelets agregometry were performed according the ISTH recommendations with (final concentrations) : ADP (2 µmol/L), epinephrin (5 mmol/L), collagen (2 µg/ml), arachidonic acid (1 mmol/L) (Helena Laboratories, Beaumont, USA), thromboxan A2 analog, U46619 (1 mmol/L) (Merck, Darmstadt, Germany), Thrombin Receptor Activating peptide (TRAP, 10 µmol/L) (Roche Rotkreuz, Switzerland). Area under curve at 4 minutes and maximal agregation were the main parametres analysed. Thrombin generation assays (TGA) were performed with PPP reagent low (phospholipids (4 µmol/L) and tissue factor (TF, 1 pmol/L); Stago, Asnières sur Seine, France) on platelet free plasma (PFP). The platelets procoagulant activity was evaluated with PRP reagent (TF 1 pmol/L) (Stago) on platelets rich plasma (PRP) and microparticles procoagulant activity with PRP reagent on plasma rich in microparticles (without platelets). After activation by Ca ionophore ?? Fibrinolysis was evaluated by a clot lysis assay determined by measurements of optical densitometry at 405 nm overtime. Coagulation was initiated by PPP reagent (Stago) sensibilised by an activator of plasminogen (t-PA, actylise®, Boehringer, France ; 200 ng/mL, final concentration)