Immune Cell Dynamics in Nordic FL Patients in the SAKK 35/10 Randomized Trial with Rituximab and Lenalidomide

• Published in: Blood 2016-12, Vol.128 (22), p.5338-5338
• Main Authors: Lockmer, Sandra, Wahlin, Björn E, Ostenstad, Bjorn, Jeppson-Ahlberg, Åsa, Sander, Birgitta, Kimby, Eva
• Format: Article
• Language: English
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V128.22.5338.5338

Background: Follicular lymphoma (FL) is the second most common lymphoma in adults. Although responsive to various therapies it is considered incurable. The CD20 antibody rituximab is well known to have improved outcome, acting through complement-mediated cytotoxicity, antibody-dependent cellular cytotoxicity (ADCC) and direct induction of apoptosis. To enhance the efficacy of rituximab different combination regimens have been used, mostly with chemotherapy but also with cytokines and immunomodulatory agents such as lenalidomide. Lenalidomide has been shown to induce durable responses with manageable toxicity in indolent lymphomas and mantle cell lymphoma. It modulates signaling pathways, enhances the capacity of T cells and natural killer (NK) cells and suppresses angiogenesis. When combined with rituximab, effects seem to be synergistic (Fowler 2014). Aim: To investigate the dynamics of immune cells in blood in patients treated with rituximab with or without lenalidomide. Patients and Methods: FL patients included in a multicenter randomized phase II trial performed by the Swiss Group for Clinical Cancer Research (SAKK) in collaboration with the Nordic Lymphoma Group (NLG) were randomized 1:1 to treatment either with rituximab alone or rituximab and lenalidomide. Inclusion criteria were histologically confirmed CD20+ FL grade 1, 2 or 3A, disease stage Ann Arbor III-IV (or II not suitable for radiotherapy) and need of therapy. In both treatment arms rituximab was administered as 4 single infusions of 375 mg/m2 weeks 1, 2, 3 and 4; patients who showed at least a minor response received 4 additional infusions at weeks 12, 13, 14 and 15. In the combination arm lenalidomide 15 mg p.o. daily was started 14 days before the first infusion and given continuously until 14 days after the last. Blood cells were sequentially sampled: at baseline, after 2 weeks’ of lenalidomide (combination arm solely), 24 hours after the first rituximab infusion and at follow-up weeks 10 and 23. Analyses of CD4+, CD8+, CD56+CD3- (NK) cells, CD19+CD20+ and CD3-CD4+ (mainly monocytes) were performed with flow cytometry and measured as percentages of mononuclear cells. T cell subpopulations CD27+CD45RA+ (naïve), CD27+CD45RO+ (memory) and CD26-CD45RA+ (effector) were measured as percentages of CD3+CD4+ and CD3+CD8+, respectively. Results: Rituximab and lenalidomide: Immune cell levels were analyzed in 13 Norwegian and Swedish patients randomized to combination treatment. Compared to basel