The B7-2 (B70) Costimulatory Molecule Expressed by Monocytes and Activated B Lymphocytes Is the CD86 Differentiation Antigen

T-cell activation is initiated after T-cell receptor binding to antigen, but also requires interactions between costimulatory molecules expressed on antigen-presenting cells. An important costimulatory molecule expressed by monocytes and activated B lymphocytes has been recently identified and terme...

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Bibliographic Details
Published in:Blood 1994-09, Vol.84 (5), p.1402-1407
Main Authors: Engel, Pablo, Gribben, John G., Freeman, Gordon J., Zhou, Liang-Ji, Nozawa, Yoshihiro, Abe, Masafumi, Nadler, Lee M., Wakasa, Haruki, Tedder, Thomas F.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antigens, CD
B-Lymphocytes - immunology
B7-1 Antigen - analysis
B7-1 Antigen - biosynthesis
B7-1 Antigen - blood
B7-2 Antigen
Biological and medical sciences
Cell Division - drug effects
CHO Cells
Cricetinae
Epitopes - analysis
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression - drug effects
Humans
Immunobiology
Immunoglobulin G
Leukocytes, Mononuclear - immunology
Lymphocyte Activation
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Membrane Glycoproteins
Muromonab-CD3 - pharmacology
Tetradecanoylphorbol Acetate - pharmacology
Thymidine - metabolism
Transfection
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Summary:T-cell activation is initiated after T-cell receptor binding to antigen, but also requires interactions between costimulatory molecules expressed on antigen-presenting cells. An important costimulatory molecule expressed by monocytes and activated B lymphocytes has been recently identified and termed B7-2 or B70. Independently, a new Cluster of Differentiation was defined in the Fifth International Leukocyte Differentiation Antigen Workshop as CD86, a molecule predominantly expressed by monocytes and activated B lymphocytes. In this study, the two monoclonal antibodies that defined CD86, FUN-1 and BU-63, were shown to bind to cDNA transfected cells expressing B7-2/B70. The FUN-1 monoclonal antibody also completely blocked the costimulatory activity of B7-2/B70 in functional assays. Therefore, the serologically defined CD86 differentiation antigen is the B7-2/B70 molecule.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V84.5.1402.1402