Polyclonal Primitive Hematopoietic Progenitors Can Be Detected in Mobilized Peripheral Blood From Patients With High-Risk Myelodysplastic Syndromes

• Published in: Blood 1995-11, Vol.86 (10), p.3660-3667
• Main Authors: Delforge, Michel, Demuynck, Hilde, Vandenberghe, Peter, Verhoef, Gregor, Zachée, Pierre, Duppen, Victor Van, Marijnen, Peter, Berghe, Herman Van den, Boogaerts, Marc A.
• Format: Article
• Language: English
• Subjects: Adult; Anemia, Refractory, with Excess of Blasts - blood; Anemia, Refractory, with Excess of Blasts - drug therapy; Anemia, Refractory, with Excess of Blasts - pathology; Anemia, Refractory, with Excess of Blasts - therapy; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Antigens, CD - analysis; Antigens, CD34 - analysis; Antigens, Differentiation, Myelomonocytic - analysis; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; Base Sequence; Biological and medical sciences; Bone Marrow - drug effects; Bone marrow, stem cells transplantation. Graft versus host reaction; Cell Separation; Cell Transformation, Neoplastic; Clone Cells - drug effects; Clone Cells - pathology; Colony-Forming Units Assay; Cytarabine - administration & dosage; Dosage Compensation, Genetic; Feasibility Studies; Female; Filgrastim; Flow Cytometry; Granulocyte Colony-Stimulating Factor - administration & dosage; Granulocyte Colony-Stimulating Factor - pharmacology; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - pathology; Humans; Lenograstim; Medical sciences; Middle Aged; Mitoxantrone - administration & dosage; Molecular Sequence Data; Recombinant Proteins - administration & dosage; Recombinant Proteins - pharmacology; Risk; Sialic Acid Binding Ig-like Lectin 3; Transfusions. Complications. Transfusion reactions. Cell and gene therapy
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V86.10.3660.bloodjournal86103660

Myelodysplasie syndromes (MDS) form a heterogeneous group of clonal hematopoietic disorders with unfavourable prognosis. Allogeneic bone marrow transplantation is the only potentially curative treatment, but remains limited to a small subgroup of younger patients with HLA-compatible donors. As autologous stem cell transplantation is currently being explored as an alternative treatment strategy for MDS, more information needs to be acquired regarding the clonal nature of the progenitor cells in these autografts. Therefore, we have analyzed the clonal patterns of highly purified hematopoietic progenitors and their mature daughter cells in mobilized peripheral blood collections procured from five female patients with high-risk MDS in complete hematologic remission. X-chromosome inactivation patterns of flowsorted immature (CD34+ 38low, CD34+ 33low ) and committed (CD34+ 38hIgh, CD34+ 33high ) progenitors were studied with the polymerase chain reaction-based HUMARA assay. In four patients, a polyclonal remission was shown in all stem cell subpopulations and their mature daughter cells whereas one patient was found to remain skewed in all fractions, except T lymphocytes. This study provides strong evidence that polyclonal immature hematopoietic progenitors can be mobilized and harvested in patients with high-risk MDS after treatment with high-dose chemotherapy.