Pegylated Human Leptin D23L Mutant—Preparation and Biological Activity In Vitro and In Vivo in Male ob /ob Mice

Recombinant monomeric human leptin (hLEP) and its D23L mutant were prepared in Escherichia coli and pegylated at their N-terminus using 20-kDa methoxy pegylated (PEG)-propionylaldehyde. As determined by both SDS-PAGE and size-exclusion chromatography, the pegylated proteins consisted of >90% mono...

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Bibliographic Details
Published in:	Endocrinology (Philadelphia) 2019-04, Vol.160 (4), p.891-898
Main Authors:	Gertler, Arieh, Solomon, Gili
Format:	Article
Language:	English
Subjects:	Animal models Animals Biological activity Blood Glucose - metabolism Body weight Body Weight - drug effects Care and treatment Circular dichroism Cytokines Dichroism E coli Eating - drug effects Endocrinology Food Food consumption Fuel consumption Gel electrophoresis Gene mutation Genetic aspects Glucose Glucose tolerance Glucose Tolerance Test Health aspects In vivo methods and tests Leptin Leptin - analogs & derivatives Leptin - metabolism Leptin - pharmacology Male Mice Mice, Obese Mutants Mutation N-Terminus Obesity Physiological aspects Polyethylene Glycols - metabolism Polyethylene Glycols - pharmacology Protein structure Reagents Receptors, Leptin - metabolism Recombinant proteins Secondary structure Size exclusion chromatography Sodium lauryl sulfate
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