Enzymatic synthesis of a novel trisaccharide, glucosyl lactoside

Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of oligosaccharides by the transglycosylation reaction with cyclomaltohexaose as a glycosyl donor and D-lactose as its acceptor. The content of oligosaccharide A as the main transfer product was 22.1 % of the tot...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1993-01, Vol.57 (1), p.56-60
Main Authors: Shibuya, T. (Hayashibara Biochemical Labs. Inc., Okayama (Japan)), Miwa, Y, Nakano, M, Yamauchi, T, Chaen, H, Sakai, S, Kurimoto, M
Format: Article
Language:English
Subjects:
alpha-Cyclodextrins
Analytical, structural and metabolic biochemistry
BACILLUS STEAROTHERMOPHILUS
Biological and medical sciences
BIOLOGICAL CONTROL
Biotechnology
Carbohydrate Sequence
Carbohydrates
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
CONTROL BIOLOGICO
Crystallization
Cyclodextrins - metabolism
ENZIMAS
ENZYME
ENZYMES
Fundamental and applied biological sciences. Psychology
Geobacillus stearothermophilus - enzymology
Glucosyltransferases - metabolism
Glycosylation
Holosides
Hydrolysis
Lactose - metabolism
LUTTE BIOLOGIQUE
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Oligosaccharides - biosynthesis
Oligosaccharides - chemistry
Other biological molecules
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
Trisaccharides - biosynthesis
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Summary:Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of oligosaccharides by the transglycosylation reaction with cyclomaltohexaose as a glycosyl donor and D-lactose as its acceptor. The content of oligosaccharide A as the main transfer product was 22.1 % of the total sugar. The content was increased to 34.1 % by hydrolysis with glucoamylase. Oligosaccharide A was isolated by column chromatography and crystallized. By chemical and enzymatic analyses, oligosaccharide A was found to be a novel non-reducing trisaccharide; O-β-D-galactopyranosyl-(1→4)-O-β-D-glucopyranosyl α-D-gluco-pyranoside.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.57.56