Development of a simple and efficient method for transformation of buckwheat plants (Fagopyrum esculentum) using Agrobacterium tumefaciens

Apical meristems of seedlings of buckwheat (Fagopyrum esculentum var. Shinano No.1) were pricked with a needle and inoculated with Agrobacterium tumefaciens (LBA4404, pBI121). The inoculated seedlings were grown to maturation and allowed to pollinate randomly to set the seeds (T1 plants). The transf...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2000-04, Vol.64 (4), p.845-847
Main Authors: Kojima, M. (Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science), Arai, Y, Iwase, N, Shirotori, K, Shioiri, H, Nozue, M
Format: Article
Language:English
Subjects:
AGROBACTERIUM TUMEFACIENS
Agrobacterium tumefaciens - genetics
apical meristem
APICAL MERISTEMS
Biological and medical sciences
Biotechnology
BREEDING METHODS
Fagopyrum - genetics
FAGOPYRUM ESCULENTUM
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
GENETIC TRANSFORMATION
Genetic Vectors - genetics
GENOMAS
GENOME
GENOMES
Glucuronidase - genetics
INOCULACION
INOCULATION
MERISTEMAS APICALES
MERISTEME APICAL
METHODE D'AMELIORATION GENETIQUE
Methods. Procedures. Technologies
METODOS DE MEJORAMIENTO GENETICO
Plants, Genetically Modified
plasmid rescue
PLASMIDE
PLASMIDIOS
PLASMIDS
TRANSFORMACION GENETICA
transformation
TRANSFORMATION GENETIQUE
Transformation, Genetic
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Apical meristems of seedlings of buckwheat (Fagopyrum esculentum var. Shinano No.1) were pricked with a needle and inoculated with Agrobacterium tumefaciens (LBA4404, pBI121). The inoculated seedlings were grown to maturation and allowed to pollinate randomly to set the seeds (T1 plants). The transformation efficiency of the T1 plants was estimated by germination in the presence of geneticin (20 mu-g/ml) and by detection of beta-glucuronidase (GUS) gene with PCR, indicating that 36% and 70% of the T1 plants were transformed, respectively. Four plants taking on a mutated morphology were selected from T1 plants which were transformed with the method using A. tumefaciens harboring a modified pBI121 for plasmid rescue. Southern blot analysis of plasmids rescued from the 4 T1 plants demonstrated that each plasmid contained a different flanking DNA of the buckwheat genome, an evidence that T-DNA was integrated in different sites of the genomic DNA among the 4 T1 plants
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.64.845