Development and Validation of a Liquid Chromatographic Method for Determination of Efavirenz in Human Plasma

A simple, rapid, and sensitive high-performance liquid chromatographic method for estimation of efavirenz in human plasma has been developed and validated. Chromatography was performed with C₁₈ analytical column and 50:50 acetonitrile-phosphate buffer (pH 3.5) as mobile phase. Compounds were monitor...

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Bibliographic Details
Published in:Chromatographia 2007-03, Vol.65 (5-6), p.359-361
Main Authors: Lakshmi Sailaja, A, Kishore Kumar, K, Ravi Kumar, D. V. R, Mohan Kumar, C, Yugandhar, N. M, Srinubabu, G
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Efavirenz
General pharmacology
Human plasma
liquid chromatography
Medical sciences
Pharmacology. Drug treatments
Validation
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Summary:A simple, rapid, and sensitive high-performance liquid chromatographic method for estimation of efavirenz in human plasma has been developed and validated. Chromatography was performed with C₁₈ analytical column and 50:50 acetonitrile-phosphate buffer (pH 3.5) as mobile phase. Compounds were monitored by UV detection at 247 nm. The retention time for efavirenz was 6.45 min and that for the internal standard, nelfinavir, was 2.042 min. Response was a linear over the concentration range of 0.1 μg-10 μg mL-¹ in human plasma. The method was simple, specific, precise and accurate and was useful for bioequivalence and pharmacokinetic studies of efavirenz.
ISSN:0009-5893
1612-1112
DOI:10.1365/s10337-006-0170-x