SPE and LC-ESI-MS for Quantitative Analysis of Mitiglinide in Human Plasma in a Bioequivalence Study

Liquid chromatography-electrospray ionization mass spectrometry has been used for rapid, selective, and sensitive quantitative analysis of mitiglinide in human plasma. Sample pretreatment involved solid-phase extraction from plasma with gliclazide as internal standard. Separation was performed on a...

Full description

Saved in:
Bibliographic Details
Published in:Chromatographia 2009-12, Vol.70 (11-12), p.1715-1719
Main Authors: Wen, Jin Hua, Xiong, Yu Qing
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Biological and medical sciences
Chemistry
Chemistry and Materials Science
Chromatography
Full Short Communication
General pharmacology
Laboratory Medicine
Medical sciences
Pharmacology. Drug treatments
Pharmacy
Proteomics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Liquid chromatography-electrospray ionization mass spectrometry has been used for rapid, selective, and sensitive quantitative analysis of mitiglinide in human plasma. Sample pretreatment involved solid-phase extraction from plasma with gliclazide as internal standard. Separation was performed on a C₁₈ column (150 × 2.0 mm) with 71:29 (v/v) acetonitrile-water (containing 0.1% formic acid and 0.2 mmol L⁻¹ ammonium acetate) as mobile phase at a flow rate of 0.2 mL min⁻¹. The method was validated then successfully applied to a clinical bioequivalence study of mitiglinide in 20 healthy volunteers after oral administration.
ISSN:0009-5893
1612-1112
DOI:10.1365/s10337-009-1358-7