Subtyping Phosphoglucomutase-1 in Semen Stains and Bloodstains: A Report on the Method

A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.2-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The g...

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Published in:Journal of forensic sciences 1986-10, Vol.31 (4), p.1341-1348
Main Authors: Budowle, B, Murch, RS, Davidson, LC, Gambel, AM, Kearney, JJ
Format: Article
Language:English
Subjects:
Blood Stains
Body fluids
Female
Forensic hematology
Forensic Medicine
Forensic sciences
Humans
Isoelectric Focusing
Male
Phosphoglucomutase - genetics
Semen - enzymology
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cited_by cdi_FETCH-LOGICAL-a414t-48aca5f07e3f87cbdd2f3a6cce7d1d18d489953fab472c5174a066f0e0a141eb3
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container_title Journal of forensic sciences
container_volume 31
creator Budowle, B
Murch, RS
Davidson, LC
Gambel, AM
Kearney, JJ
description A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.2-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The gel contained 1.2% (w/v) N-(2-hydroxyethyl) piperazine-N-3-propanesulfonic acid (EPPS) and pH 5 to 7 ampholytes (4% w/v). When maintained at room temperature, laboratory-prepared bloodstains and semen stains could be typed for phosphoglucomutase-1 up to four months and three weeks, respectively. An evaluation of phosphoglucomutase-1 typing by isoelectric focusing and the Group I system was performed on casework samples submitted to the FBI Laboratory. In addition to the increased discriminating probability of phosphoglucomutase-1 when subtyped, isoelectric focusing yielded an increase in positive calls on questioned bloodstains (65.6 versus 36.2%) and dried seminal stains (16.4 versus 13.1%) compared with the Group I system.
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Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.2-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The gel contained 1.2% (w/v) N-(2-hydroxyethyl) piperazine-N-3-propanesulfonic acid (EPPS) and pH 5 to 7 ampholytes (4% w/v). When maintained at room temperature, laboratory-prepared bloodstains and semen stains could be typed for phosphoglucomutase-1 up to four months and three weeks, respectively. An evaluation of phosphoglucomutase-1 typing by isoelectric focusing and the Group I system was performed on casework samples submitted to the FBI Laboratory. 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This material may not be reproduced or copied, in whole or in part, in any printed, mechanical, electronic, film, or other distribution and storage media, without the written consent of the publisher.</rights><rights>Copyright American Society for Testing and Materials Oct 1986</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a414t-48aca5f07e3f87cbdd2f3a6cce7d1d18d489953fab472c5174a066f0e0a141eb3</citedby><cites>FETCH-LOGICAL-a414t-48aca5f07e3f87cbdd2f3a6cce7d1d18d489953fab472c5174a066f0e0a141eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,9771,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2946809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Budowle, B</creatorcontrib><creatorcontrib>Murch, RS</creatorcontrib><creatorcontrib>Davidson, LC</creatorcontrib><creatorcontrib>Gambel, AM</creatorcontrib><creatorcontrib>Kearney, JJ</creatorcontrib><title>Subtyping Phosphoglucomutase-1 in Semen Stains and Bloodstains: A Report on the Method</title><title>Journal of forensic sciences</title><addtitle>J Forensic Sci</addtitle><description>A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. 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source ASTM Journals
subjects Blood Stains
Body fluids
Female
Forensic hematology
Forensic Medicine
Forensic sciences
Humans
Isoelectric Focusing
Male
Phosphoglucomutase - genetics
Semen - enzymology
title Subtyping Phosphoglucomutase-1 in Semen Stains and Bloodstains: A Report on the Method
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