Isolation and characterization of 'Rep' PepYLCV encoding gene from West Sumatra

Replicase ('Rep') protein of Geminivirus is known as one of the important components not only for its successful replication in their host but also known to interact with various host plant proteins. However, it is still unclear if those interactions are associated with symptoms level. Thi...

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Bibliographic Details
Published in:Plant omics 2018-02, Vol.11 (1), p.37-41
Main Authors: Nova, Bastian, Syafriani, Elly, Asben, Alfi, Jamsari, Jamsari
Format: Article
Language:English
Subjects:
Analysis
DNA
Methodology
Universitas Andalas
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Summary:Replicase ('Rep') protein of Geminivirus is known as one of the important components not only for its successful replication in their host but also known to interact with various host plant proteins. However, it is still unclear if those interactions are associated with symptoms level. This research aims to explore the possibility of Rep as pathogenic determinant by in silico approach. Here we report the comparison of three Rep sequences isolated from Pesisir Selatan and Tanah Datar districts in West Sumatra Indonesia. The PCR-based cloning approach was used in this study to isolate the gene sequences from all isolates. Pathogenic determinant was predicted from phenotype and genotype analysis. Phenotype data showed symptoms appearance after 8 dpi for PSSWS14 and 20 dpi for PSSWS3. Furthermore, genotype showed that the nonconserved region in N-terminal of 'Rep' makes different in its putative binding site. It is prospective to be related to the symptoms appearance rates. We predict the differences in N-terminal of Rep affecting the symptoms appearance rates of Geminivirus infection.
ISSN:1836-0661
1836-3644
DOI:10.21475/poj.11.01.18.pne1055