Serum levels of IL-33 and its receptor ST2 are elevated in patients with ankylosing spondylitis

Objectives: The aims of this study were to measure the levels of interleukin (IL)-33 and ST2 and T-helper (Th)2-associated cytokines (IL-13, IL-4, IL-5) in patients with ankylosing spondylitis (AS), and examine the correlation of serum cytokine levels with disease activity and laboratory parameters....

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Bibliographic Details
Published in:Scandinavian journal of rheumatology 2013-01, Vol.42 (3), p.226-231
Main Authors: Li, G-X, Wang, S, Duan, Z-H, Zeng, Z, Pan, F-M
Format: Article
Language:English
Subjects:
Adult
Case-Control Studies
Female
Humans
Interleukin-1 Receptor-Like 1 Protein
Interleukin-33
Interleukins - blood
Male
Real-Time Polymerase Chain Reaction
Receptors, Cell Surface - blood
RNA, Messenger - blood
Spondylitis, Ankylosing - blood
Th2 Cells - metabolism
Young Adult
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Summary:Objectives: The aims of this study were to measure the levels of interleukin (IL)-33 and ST2 and T-helper (Th)2-associated cytokines (IL-13, IL-4, IL-5) in patients with ankylosing spondylitis (AS), and examine the correlation of serum cytokine levels with disease activity and laboratory parameters. Method: Serum IL-33, IL-13, IL-4, and IL-5 levels were assessed by sandwich enzyme-linked immunosorbent assay (ELISA), and the mRNA levels of IL-33 and ST2 were quantified by real-time quantitative polymerase chain reaction (RT-qPCR), in 43 AS samples and compared with 27 age- and sex-matched healthy controls. Results: Serum IL-33, IL-13, and IL-4 levels were increased significantly in AS patients compared with controls (p < 0.01); moreover, serum IL-33 and IL-13 levels were significantly higher in patients with active AS than in those with inactive AS (p < 0.05). The serum levels of IL-5 showed no significant difference between AS patients and controls (p > 0.05). Serum IL-33 levels were positively correlated with both IL-13 (r = 0.306, p < 0.01) and IL-4 levels (r = 0.432, p < 0.01). The mRNA levels of IL-33 and ST2 were significantly different between AS patients and controls (p < 0.01) but not between active and inactive AS patients. Conclusions: Serum levels of IL-33 could partially reflect AS disease activity and indicate that IL-33/ST2 signalling plays an important role in the pathogenesis of AS.
ISSN:0300-9742
1502-7732
DOI:10.3109/03009742.2012.735700