Measurement of sVEGF R1 and PlGF in Serum: Comparing Prototype Assays from Beckman Coulter, Inc. to R&D Systems Microplate Assays

Objective. To compare the performance of prototype Access® sVEGF R1 and PlGF automated immunoassays from Beckman Coulter to the Quantikine® microplate ELISA assays by R&D Systems. Methods. Samples obtained from pregnant women, non-pregnant women and men were assayed according to manufacturers�...

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Bibliographic Details
Published in:Hypertension in pregnancy 2011-02, Vol.30 (1), p.18-27
Main Authors: Wothe, Donald, Gaziano, Emanuel, Sunderji, Shiraz, Romero, Roberto, Kusanovic, Juan Pedro, Rogers, Linda, Hodges-Savola, Cheryl, Roberts, Sean, Wassenberg, James
Format: Article
Language:English
Subjects:
Adult
Angiogenesis
Female
Humans
Immunoassay - methods
Male
Placenta Growth Factor
Placental Growth Factor
Pre-Eclampsia - blood
Pre-Eclampsia - diagnosis
Preeclampsia
Pregnancy
Pregnancy Proteins - blood
Sensitivity and Specificity
sFlt-1
Soluble Vascular Endothelial Growth Factor
Vascular Endothelial Growth Factor Receptor-1 - blood
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Summary:Objective. To compare the performance of prototype Access® sVEGF R1 and PlGF automated immunoassays from Beckman Coulter to the Quantikine® microplate ELISA assays by R&D Systems. Methods. Samples obtained from pregnant women, non-pregnant women and men were assayed according to manufacturers' instructions. Results. Compared to the Quantikine assays, the Access assays demonstrated improved precision, increased sensitivity, broader dynamic ranges, and reduced analysis time. The Access assays were found to be specific for free sVEGF R1 and free PlGF. Conclusion. There was good correlation between the Access and Quantikine assays. Superior performance by Access assays may have important prenatal diagnostic implications.
ISSN:1064-1955
1525-6065
DOI:10.3109/10641950903322881