Development and characterization of multiple emulsions for controlled release of Trichilia catigua (Catuaba) extract

• Published in: Pharmaceutical development and technology 2016-12, Vol.21 (8), p.933-942
• Main Authors: Lonni, Audrey Alesandra Stinghen Garcia, Munhoz, Vanessa Marquito, Lopes, Gisely Cristiny, Longhini, Renata, Borghi-Pangoni, Fernanda Belincanta, dos Santos, Rafaela Said, Junqueira, Mariana Volpato, Natali, Maria Raquel Marçal, Leite-Mello, Eneri, Guimaraes, Francine Baesso, Baesso, Mauro Luciano, Scarminio, Ieda Spacino, Bruschi, Marcos Luciano, Mello, João Carlos Palazzo de
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant activity; Antioxidants - chemistry; Canola Oil; Chemistry, Pharmaceutical - methods; Delayed-Action Preparations - chemistry; drug delivery system; Drug Liberation; drug release; Drug Stability; Emulsions - chemistry; Meliaceae - chemistry; Particle Size; Permeability; Plant Oils - chemistry; Rheology; rheometry; Skin - metabolism; Skin Absorption; stability; Swine; Water - chemistry
• ISSN: 1083-7450; 1097-9867
• DOI: 10.3109/10837450.2015.1081611

Considering the antioxidant activity of the Trichilia catigua extract (TCE), the aim of the current study was to develop and characterize W/O/W multiple emulsions containing different vegetable oils as a platform to deliver a TCE. The extract displayed antioxidant activity (IC 50 ) of 4.59 µg/mL and total phenol content (TPC) of 50.84%. Formulations were prepared by the phase-inversion emulsification method and analyzed for morphological appearance, pH, conductivity, droplet size and distribution, content of active, rheological properties, in vitro release, skin permeation, and stability. Formulations prepared with canola oil were selected and displayed regular morphology, mean diameter 2.77 µm (without TCE), 3.07 µm with 0.5% and 3.23 µm with 1.0% TCE. Rheometry (flow) showed pseudoplastic behavior with minimal thixotropy for both systems. TCE could be released from emulsions containing 1.0% and 0.5% TCE in a controlled manner for 16 and 23 h, respectively. The emulsions allowed good retention of TCE in the skin (stratum corneum, epidermis, and dermis). In a 180-d assessment of accelerated chemical stability, TPC was more reduced for the emulsions at 40 °C; other parameters remained stable. Multiple emulsions containing TCE were developed, exhibited good characteristics, and may be considered for future investigations as anti-aging formulations for the skin.