hTERT methylation and expression in gastric cancer

Gastric cancer is the second most prevalent cause of cancer death worldwide. DNA methylation is a common event in gastric carcinogenesis. hTERT seems to be the rate-limiting determinant of telomerase activation, which is responsible for stability and life span. hTERT hypermethylation has been associ...

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Bibliographic Details
Published in:Biomarkers 2009-12, Vol.14 (8), p.630-636
Main Authors: Gigek, Carolina Oliveira, Leal, Mariana Ferreira, Silva, Patricia Natalia Oliveira, Lisboa, Luara Carolina Frias, Lima, Eleonidas Moura, Calcagno, Danielle Queiroz, Assumpção, Paulo Pimentel, Burbano, Rommel Rodriguez, Smith, Marilia de Arruda Cardoso
Format: Article
Language:English
Subjects:
Adult
Biomarkers, Tumor - metabolism
DNA Methylation
Female
Gastric cancer
Gastric Mucosa - metabolism
Gene Expression Regulation, Neoplastic
hTERT
Humans
IHC
Male
methylation
Middle Aged
MSP
Promoter Regions, Genetic
protein expression
Stomach Neoplasms - genetics
Telomerase - genetics
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Summary:Gastric cancer is the second most prevalent cause of cancer death worldwide. DNA methylation is a common event in gastric carcinogenesis. hTERT seems to be the rate-limiting determinant of telomerase activation, which is responsible for stability and life span. hTERT hypermethylation has been associated with telomerase expression. In the present study, we investigated the promoter methylation status and hTERT protein expression in gastric cancer and normal mucosa samples. One hundred and nine gastric cancer and 53 normal mucosa samples were investigated through methylation-specific PCR. Immunohistochemistry was analysed using peroxidase in 55 gastric cancer and 18 normal gastric mucosa samples. This is the first study evaluating hTERT methylation status in gastric carcinogenesis. We did not observe hTERT protein expression in normal gastric mucosa. Moreover, hTERT expression was observed in 80% of tumours and was associated with gastric cancer (p 
ISSN:1354-750X
1366-5804
DOI:10.3109/13547500903225912