Dissection of the SHP-1 functions in FcεRI signaling (139.8)

• Published in: The Journal of immunology (1950) 2009-04, Vol.182 (1_Supplement), p.139-139.8
• Main Authors: Mizuno, Kazuya, Nakata, Kazuko, Yakura, Hidetaka
• Format: Article
• Language: English
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.182.Supp.139.8

Although SHP-1, a cytoplasmic protein tyrosine phosphatase (PTP), is shown to be a negative modulator in hematopoietic cells, its precise molecular mechanisms are not well defined. To clarify how SHP-1 regulates FcεRI-mediated signaling in mast cells, we generated bone marrow-derived mast cells (BMMC) from motheaten (me) that lack SHP-1 expression, viable motheaten (mev) that express phosphatase-deficient form of SHP-1, and control (WT) mice and examined downstream signaling events and final outputs following FcεRI crosslinking. FcεRI-induced tyrosine phosphorylation of SLP-76 and LAT, activation of MAPKs, and cytokine production were increased in me- and mev-derived BMMC, in good accordance with the previous implication of SHP-1 as a negative regulator. Since the activity of Syk that is responsible for the phosphorylation of SLP-76 and LAT was comparable in all BMMCs, both molecules might be substrates of SHP-1 in mast cells. In contrast, me-BMMC showed severely impaired PLCγ phosphorylation, FcεRI-induced calcium mobilization, especially extracellular calcium entry, and degranulation. We could not observe these phenotypes in mev-BMMC, thus suggesting that the expression of SHP-1, irrespective PTP activity, is sufficient for the positive regulation of these pathways. The absence of SHP-1 expression disrupted the association between SLP-76 and PLCγ, which might explain, at least in part, the decrease of calcium response in me-BMMC. Collectively, all these results suggest that SHP-1 regulates FcεRI-induced downstream signaling events both negatively and positively by functioning as a PTP and an adaptor protein contributing to the formation of signaling complex, respectively.