Characterization of Vibrio parahaemolyticus serotype O3:K6 activation of murine macrophage. (111.27)

• Published in: The Journal of immunology (1950) 2011-04, Vol.186 (1_Supplement), p.111-111.27
• Main Authors: Waters, Stephanie, Parent, Michelle, Blumerman, Seth, Whitaker, Brian, Joerger, Torsten, Boyd, Ethna
• Format: Article
• Language: English
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.186.Supp.111.27

Vibrio parahaemolyticus is the leading cause of seafood-related bacterial gastroenteritis in the United States. There is a dearth of literature regarding the host response to, and subsequent elimination of, infection with this pathogen. Here, we have developed an in vitro model using the virulent clinical isolate V. parahaemolyticus RIMD2210633, an O3:K6 serotype. We determined that this isolate could infect RAW264.7 cells, a murine macrophage cell line and bone marrow derived macrophage (BMDM), resulting in the production of the early proinflammatory cytokines interleukin-1 alpha (IL-1α), interleukin 6 (IL-6) and interleukin 10 (IL-10). Additionally, infected RAW264.7 macrophage displayed increased surface CD86, CD40 and class II MHC expression while displaying a decrease in TLR4. Concurrently, we investigated the production of reactive oxygen species (ROS) by both RAW264.7 and BMDM infected with this virulent clinical strain. We report that in response to infection, both types of macrophage demonstrate increased production of ROS. We have determined that infection of macrophage by a virulent clinical isolate of Vibrio parahaemolyticus results in immune activation as demonstrated by increased cell surface molecules and proinflammatory cytokine production. Characterization of this response will provide the foundation to understand elimination of this organism from the infected host.