Proteomic analysis of exosomes released from Leishmania infected macrophages

• Published in: The Journal of immunology (1950) 2020-05, Vol.204 (1_Supplement), p.156-156.19
• Main Authors: Keller, M Murphy, Kelly, Patrick H, Pope, R Marshall, Thery, Fabien, Wilson, Mary E
• Format: Article
• Language: English
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.204.Supp.156.19

Visceral leishmaniasis (VL) is a potentially fatal disease caused by Leishmania donovani or L. infantum. Parasites reside quiescently in macrophages (Mϕ) but dramatically affect systemic immune responses. These Mϕs, like most eukaryotic cells, release small, protein- and RNA-laden extracellular vesicles called exosomes, which facilitate intercellular communication. We hypothesized exosomes released from infected Mϕs mediate some systemic immune changes in VL. If correct, exoproteomes of infected Mϕs must differ from uninfected Mϕs. Monocyte derived Mϕs (MDMs) from 4 healthy human donors were infected with L. infantum and extracellular parasites removed. Exosomes were collected and exoproteomes were isotope labeled and identified by LC-MS/MS. Data were exported in MaxQuant 1.6.3.3, and analyzed with Perseus 1.6.2.3. After filtering for quality and for proteins found in all 5 technical replicates of at least one group, 244 proteins were quantified. Pairwise comparison revealed significant differences between donors (FDR 0.05, S0=1), and between infected and uninfected states. 2-way ANOVA (p