Bactericidal, Bacteriolytic, and Antibacterial Virulence Activities of Boesenbergia pandurata (Roxb) Schltr Extract against Streptococcus pyogenes

Purpose: To determine the anti- Streptococcus pyogenes activity of the chloroform extract of Boesenbergia pandurata (Roxb.) Schltr. (Zingiberaceae) and investigate its possible antibacterial mechanisms of action. Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration...

Full description

Saved in:
Bibliographic Details
Published in:Tropical journal of pharmaceutical research 2014-01, Vol.12 (6), p.1023
Main Authors: Limsuwan, Surasak, Voravuthikunchai, Supayang Piyawan
Format: Article
Language:English
Subjects:
Antibacterial
Bacteriocidal
Bacteriolytic
Boesenbergia pandurata
Streptococcus pyogenes
Virulence
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose: To determine the anti- Streptococcus pyogenes activity of the chloroform extract of Boesenbergia pandurata (Roxb.) Schltr. (Zingiberaceae) and investigate its possible antibacterial mechanisms of action. Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were investigated against 47 clinical isolates of S. pyogenes. Time-kill study was performed to determine how quickly the extract acts on S. pyogenes. The ability of the extract to damage bacterial cell wall and effects on S. pyogenes virulence factors including protease enzyme and haemolysin were investigated. Results: The extract exhibited good antibacterial activity against all of the clinical isolates of S. pyogenes with similar MIC and MBC ranging from 3.91-62.50 µg/ml. Complete killing of the bacterial cells by the extract at concentrations of 4MIC, 2MIC, and MIC occurred within 4, 8, and 12 h, respectively. Treatment of the bacterial cells with the extract at 2MIC and 4MIC caused cell lysis. All the test concentrations (1/32 - 1/2MIC) produced no effects on protease and haemolysin enzymes. Conclusion: Boesenbergia pandurata extract has powerful in vitro activity against S. pyogenes. The ability of the extract to lyse the bacterial cells suggests that the mechanism of action may be associated with cell wall and cell membrane damage.
ISSN:1596-5996
1596-9827
DOI:10.4314/tjpr.v12i6.23