Evaluation of radical scavenging properties and the protective role of papaya fruits extracts against oxidative stress in rats fed aflatoxin-contaminated diet

• Published in: Comunicata Scientiae 2013, Vol.4 (1), p.43-57
• Main Authors: Salah Hassan, Nabila, Abdel-Salam El-Nekeety, Aziza, Ashery Khadrawy, Yasser, Gmalel-din Abdel-Wahhab, Khaled, Abdel-Wahed Mannaa, Fathia, Attia Abdel-Wahhab, Mosaad
• Format: Article
• Language: eng ; por
• Subjects: aflatoxinas; Aflatoxine; Aflatoxins; Aflatóxinas; antioxidant; antioxidantes; Carica papaya; estresse oxidativo; http://aims.fao.org/aos/agrovoc/c_8638; micotoxinas; mycotoxins; oxidative stress; أفلاتوكسين; 黄曲霉毒素
• ISSN: 2177-5133; 2177-5133

This study aimed to determine the total phenols and DPPH scavenging properties of aqueous (AE) and ethanolic (EE) extracts of papaya fruits in vitro and to evaluate their hepatoprotective effects against aflatoxicosis in vivo. Sixty female Sprague-Dawley rats were divided into six groups and treated for 4 weeks including the control group, the group fed aflatoxins-contaminated diet (2 mg/ kg diet); the group treated orally with EE (250 mg/kg b.w); the group treated orally with AE (250 mg/ kg b.w); the groups fed AFs-contaminated diet and treated with EE or AE. At the end of the treatment period, blood and liver samples were collected for biochemical, histological and histochemical study. The results revealed that AE has higher total phenol content and DPPH scavenging activity if compared to EE. The in vivo results indicated that animals fed AFs-contaminated diet showed significant biochemical, histological and histochemical changes typical to those reported in the literature. Animals treated with the extracts and AFs showed a significant improvement in all biochemical parameters, histological and histochemical picture of the liver. This improvement was more pronounced in the group treated with AE. It could be concluded that papaya fruits can be used as a functional dietary ingredient to reduce hepatotoxicity. Este estudo teve como objetivo determinar o teor de fenóis e propriedades de remoção de DPPH em extratos aquosos (AE) e etanólicos (EE) de frutos de mamão in vitro e avaliar seus efeitos hepatoprotetores contra aflatoxicose in vivo. Sessenta fêmeas Sprague-Dawley foram divididas em seis grupos e foram tratadas durante 4 semanas, sendo o grupo de controle, o grupo alimentado com a dieta contaminada com aflatoxinas-(2 mg / kg de dieta), o grupo tratado oralmente com EE (250 mg / kg de peso corporal); o grupo tratado por via oral com AE (250 mg / kg de peso corporal), os grupos alimentados com dieta AFS contaminado e tratado com EE ou AE. No final do período de tratamento, as amostras de sangue e do fígado foram colhidas para estudos bioquímicos, histológicos e histoquímicos. Os resultados revelaram que AE tem maior teor de fenóis totais e atividade DPPH se comparado a EE. Os resultados in vivo indicaram que os animais alimentados com a dieta AFs contaminado mostraram significativas alterações bioquímicas, histológicas e histoquímicas, típicas dos relatos na literatura. Os animais tratados com os extratos e AFS apresentaram uma melhora significativa em t