Glu-108 in Saccharomyces cerevisiae Rad51 Is Critical for DNA Damage-Induced Nuclear Function

• Published in: mSphere 2019-03, Vol.4 (2)
• Main Authors: Suhane, Tanvi, Bindumadhavan, Vijayalakshmi, Fangaria, Nupur, Nair, Achuthsankar S, Tabassum, Wahida, Muley, Poorvaja, Bhattacharyya, Mrinal K, Bhattacharyya, Sunanda
• Format: Article
• Language: English
• Subjects: Adenosine triphosphatase; Amino acids; Animals; Bioinformatics; Cell cycle; Chromatin; Computational Biology; Deficient mutant; Deoxyribonucleic acid; DNA; DNA Damage; DNA Repair; DNA, Fungal - genetics; DNA-Binding Proteins - genetics; Double-strand break repair; Epistasis; Gene conversion; Homologous recombination; Hsp90; HSP90 Heat-Shock Proteins - genetics; HSP90 Heat-Shock Proteins - metabolism; Hsp90 protein; Hsp90-Rad51 interaction; Humans; Life sciences; Methyl methanesulfonate; Methyl Methanesulfonate - pharmacology; Mice; Molecular Biology and Physiology; molecular chaperone; Mutation; Phenotypes; Proteasomes; Protein Binding; Protein interaction; Proteins; Rad51 Recombinase - genetics; Rad51 Recombinase - metabolism; Rad51 recruitment to chromatin; Rad52 DNA Repair and Recombination Protein - genetics; Rad52 protein; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Studies; Yeast
• ISSN: 2379-5042; 2379-5042
• DOI: 10.1128/mSphere.00082-19

DNA damage-induced Rad51 focus formation is the hallmark of homologous recombination-mediated DNA repair. Earlier, we reported that Rad51 physically interacts with Hsp90, and under the condition of Hsp90 inhibition, it undergoes proteasomal degradation. Here, we show that the dynamic interaction between Rad51 and Hsp90 is crucial for the DNA damage-induced nuclear function of Rad51. Guided by a bioinformatics study, we generated a single mutant of Rad51, which resides at the N-terminal domain, outside the ATPase core domain. The mutant with an E to L change at residue 108 (Rad51 ) was predicted to bind more strongly with Hsp90 than the wild-type (Rad51 ). A coimmunoprecipitation study demonstrated that there exists a distinct difference between the associations of Rad51 -Hsp90 and of Rad51 -Hsp90. We found that upon DNA damage, the association between Rad51 and Hsp90 was significantly reduced compared to that in the undamaged condition. However, the mutant Rad51 remained tightly associated with Hsp90 even after DNA damage. Consequently, the recruitment of Rad51 to the double-stranded broken ends was reduced significantly. The - strain manifested severe sensitivity toward methyl methanesulfonate (MMS) and a complete loss of gene conversion efficiency, a phenotype similar to that of the strain. Previously, some of the N-terminal domain mutants of Rad51 were identified in a screen for a Rad51 interaction-deficient mutant; however, our study shows that Rad51 is not defective either in the self-interaction or its interaction with the members of the Rad52 epistatic group. Our study thus identifies a novel mutant of Rad51 which, owing to its greater association with Hsp90, exhibits a severe DNA repair defect. Rad51-mediated homologous recombination is the major mechanism for repairing DNA double-strand break (DSB) repair in cancer cells. Thus, regulating Rad51 activity could be an attractive target. The sequential assembly and disassembly of Rad51 to the broken DNA ends depend on reversible protein-protein interactions. Here, we discovered that a dynamic interaction with molecular chaperone Hsp90 is one such regulatory event that governs the recruitment of Rad51 onto the damaged DNA. We uncovered that Rad51 associates with Hsp90, and upon DNA damage, this complex dissociates to facilitate the loading of Rad51 onto broken DNA. In a mutant where such dissociation is incomplete, the occupancy of Rad51 at the broken DNA is partial, which results in inefficient DNA re