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A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum
The aim of the present study was to detect natural infection by Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in Barcarena, state of Pará, Brazil, through the use of three primer sets. With this approach, it is unnecessary to previously dissect the sandfly specimens. DNA of 280...
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| Published in: | Memórias do Instituto Oswaldo Cruz 2014-07, Vol.109 (4), p.442-447 |
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| cites | cdi_FETCH-LOGICAL-b537t-449191b05d068659e097f15ed1f9441ae84cf7e58441b568fc56a5aabb8522da3 |
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| container_title | Memórias do Instituto Oswaldo Cruz |
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| creator | Freitas-Lidani, Kárita Cláudia de Messias-Reason, Iara J Ishikawa, Edna Aoba Y |
| description | The aim of the present study was to detect natural infection by
Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in
Barcarena, state of Pará, Brazil, through the use of three primer
sets. With this approach, it is unnecessary to previously dissect the
sandfly specimens. DNA of 280 Lu. longipalpis female specimens were
extracted from the whole insects. PCR primers for kinetoplast
minicircle DNA (kDNA), the mini-exon gene and the small subunit
ribosomal RNA (SSU-rRNA) gene of Leishmania were used, generating
fragments of 400 bp, 780 bp and 603 bp, respectively. Infection by the
parasite was found with the kDNA primer in 8.6% of the cases, with the
mini-exon gene primer in 7.1% of the cases and with the SSU-rRNA gene
primer in 5.3% of the cases. These data show the importance of
polymerase chain reaction as a tool for investigating the molecular
epidemiology of visceral leishmaniasis by estimating the risk of
disease transmission in endemic areas, with the kDNA primer
representing the most reliable marker for the parasite. |
| doi_str_mv | 10.1590/0074-0276130285 |
| format | article |
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Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in
Barcarena, state of Pará, Brazil, through the use of three primer
sets. With this approach, it is unnecessary to previously dissect the
sandfly specimens. DNA of 280 Lu. longipalpis female specimens were
extracted from the whole insects. PCR primers for kinetoplast
minicircle DNA (kDNA), the mini-exon gene and the small subunit
ribosomal RNA (SSU-rRNA) gene of Leishmania were used, generating
fragments of 400 bp, 780 bp and 603 bp, respectively. Infection by the
parasite was found with the kDNA primer in 8.6% of the cases, with the
mini-exon gene primer in 7.1% of the cases and with the SSU-rRNA gene
primer in 5.3% of the cases. These data show the importance of
polymerase chain reaction as a tool for investigating the molecular
epidemiology of visceral leishmaniasis by estimating the risk of
disease transmission in endemic areas, with the kDNA primer
representing the most reliable marker for the parasite.</description><identifier>ISSN: 1678-8060</identifier><identifier>ISSN: 0074-0276</identifier><identifier>EISSN: 1678-8060</identifier><identifier>DOI: 10.1590/0074-0276130285</identifier><identifier>PMID: 25004147</identifier><language>eng</language><publisher>Brazil: Fundação Oswaldo Cruz, Fiocruz</publisher><subject>Animals ; DNA Primers - genetics ; DNA, Kinetoplast - genetics ; DNA, Protozoan - genetics ; Female ; Genetic Markers ; Insect Vectors - classification ; Insect Vectors - parasitology ; Leishmania (Leishmania) infantum ; Leishmania infantum - genetics ; Leishmania infantum - isolation & purification ; Lutzomyia longipalpis ; PARASITOLOGY ; Polymerase Chain Reaction ; Psychodidae - classification ; Psychodidae - parasitology ; Sensitivity and Specificity ; TROPICAL MEDICINE ; visceral leishmaniasis</subject><ispartof>Memórias do Instituto Oswaldo Cruz, 2014-07, Vol.109 (4), p.442-447</ispartof><rights>Copyright 2014 - Memórias do Instituto Oswaldo Cruz</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b537t-449191b05d068659e097f15ed1f9441ae84cf7e58441b568fc56a5aabb8522da3</citedby><cites>FETCH-LOGICAL-b537t-449191b05d068659e097f15ed1f9441ae84cf7e58441b568fc56a5aabb8522da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155845/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155845/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,24150,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25004147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Freitas-Lidani, Kárita Cláudia</creatorcontrib><creatorcontrib>de Messias-Reason, Iara J</creatorcontrib><creatorcontrib>Ishikawa, Edna Aoba Y</creatorcontrib><title>A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum</title><title>Memórias do Instituto Oswaldo Cruz</title><addtitle>Mem Inst Oswaldo Cruz</addtitle><description>The aim of the present study was to detect natural infection by
Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in
Barcarena, state of Pará, Brazil, through the use of three primer
sets. With this approach, it is unnecessary to previously dissect the
sandfly specimens. DNA of 280 Lu. longipalpis female specimens were
extracted from the whole insects. PCR primers for kinetoplast
minicircle DNA (kDNA), the mini-exon gene and the small subunit
ribosomal RNA (SSU-rRNA) gene of Leishmania were used, generating
fragments of 400 bp, 780 bp and 603 bp, respectively. Infection by the
parasite was found with the kDNA primer in 8.6% of the cases, with the
mini-exon gene primer in 7.1% of the cases and with the SSU-rRNA gene
primer in 5.3% of the cases. These data show the importance of
polymerase chain reaction as a tool for investigating the molecular
epidemiology of visceral leishmaniasis by estimating the risk of
disease transmission in endemic areas, with the kDNA primer
representing the most reliable marker for the parasite.</description><subject>Animals</subject><subject>DNA Primers - genetics</subject><subject>DNA, Kinetoplast - genetics</subject><subject>DNA, Protozoan - genetics</subject><subject>Female</subject><subject>Genetic Markers</subject><subject>Insect Vectors - classification</subject><subject>Insect Vectors - parasitology</subject><subject>Leishmania (Leishmania) infantum</subject><subject>Leishmania infantum - genetics</subject><subject>Leishmania infantum - isolation & purification</subject><subject>Lutzomyia longipalpis</subject><subject>PARASITOLOGY</subject><subject>Polymerase Chain Reaction</subject><subject>Psychodidae - classification</subject><subject>Psychodidae - parasitology</subject><subject>Sensitivity and Specificity</subject><subject>TROPICAL MEDICINE</subject><subject>visceral leishmaniasis</subject><issn>1678-8060</issn><issn>0074-0276</issn><issn>1678-8060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVUk1vEzEQXSEQLYEzN-RjOaS1vfZ-XJCqipZKkTgAZ2vWO5s4eO1ge4vSX4-XtGkrWfJ4_N6bz6L4yOg5ky29oLQWS8rripWUN_JVccqqulk2tKKvn9knxbsYtzQDy0q8LU64pFQwUZ8W95dE-3EHwUTviB_I6C3qyUIgI4TfGCJJnvSYUCeymtK9H_cGiPVubXZgdyYSB2kKYO2eGDdkGPbkr0kbskITNyO4DD97sj_PKHBpGt8XbwawET883Ivi1_XXn1fflqvvN7dXl6tlJ8s6LYVoWcs6KntaNZVskbb1wCT2bGiFYICN0EONssmPTlbNoGUFEqDrGsl5D-WiuD3o9h62ahdMLmyvPBj13-HDWkFIRltUlMFM5MgZncNCCS0y0KIsWy4Rs9b5QStqg9arrZ-Cy8mrH_Mk1DwJTpmgub35CJ4JXw6E3dSN2Gt0KffqRRYvf5zZqLW_U4LJXJLMAmcPAsH_mTAmNZqo0Vpw6KeoMoxRLpu8AYvi4gDVwccYcDiGYVTN66KOSR7WJTM-Pc_uiH_cj6d6O-OtcXhE6GBAPTq9zidXXVXlPxL6yxQ</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Freitas-Lidani, Kárita Cláudia</creator><creator>de Messias-Reason, Iara J</creator><creator>Ishikawa, Edna Aoba Y</creator><general>Fundação Oswaldo Cruz, Fiocruz</general><general>Instituto Oswaldo Cruz, Ministério da Saúde</general><general>Fundação Oswaldo Cruz (FIOCRUZ)</general><scope>RBI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20140701</creationdate><title>A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum</title><author>Freitas-Lidani, Kárita Cláudia ; de Messias-Reason, Iara J ; Ishikawa, Edna Aoba Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b537t-449191b05d068659e097f15ed1f9441ae84cf7e58441b568fc56a5aabb8522da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>DNA Primers - genetics</topic><topic>DNA, Kinetoplast - genetics</topic><topic>DNA, Protozoan - genetics</topic><topic>Female</topic><topic>Genetic Markers</topic><topic>Insect Vectors - classification</topic><topic>Insect Vectors - parasitology</topic><topic>Leishmania (Leishmania) infantum</topic><topic>Leishmania infantum - genetics</topic><topic>Leishmania infantum - isolation & purification</topic><topic>Lutzomyia longipalpis</topic><topic>PARASITOLOGY</topic><topic>Polymerase Chain Reaction</topic><topic>Psychodidae - classification</topic><topic>Psychodidae - parasitology</topic><topic>Sensitivity and Specificity</topic><topic>TROPICAL MEDICINE</topic><topic>visceral leishmaniasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freitas-Lidani, Kárita Cláudia</creatorcontrib><creatorcontrib>de Messias-Reason, Iara J</creatorcontrib><creatorcontrib>Ishikawa, Edna Aoba Y</creatorcontrib><collection>Bioline International</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freitas-Lidani, Kárita Cláudia</au><au>de Messias-Reason, Iara J</au><au>Ishikawa, Edna Aoba Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum</atitle><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle><addtitle>Mem Inst Oswaldo Cruz</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>109</volume><issue>4</issue><spage>442</spage><epage>447</epage><pages>442-447</pages><issn>1678-8060</issn><issn>0074-0276</issn><eissn>1678-8060</eissn><abstract>The aim of the present study was to detect natural infection by
Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in
Barcarena, state of Pará, Brazil, through the use of three primer
sets. With this approach, it is unnecessary to previously dissect the
sandfly specimens. DNA of 280 Lu. longipalpis female specimens were
extracted from the whole insects. PCR primers for kinetoplast
minicircle DNA (kDNA), the mini-exon gene and the small subunit
ribosomal RNA (SSU-rRNA) gene of Leishmania were used, generating
fragments of 400 bp, 780 bp and 603 bp, respectively. Infection by the
parasite was found with the kDNA primer in 8.6% of the cases, with the
mini-exon gene primer in 7.1% of the cases and with the SSU-rRNA gene
primer in 5.3% of the cases. These data show the importance of
polymerase chain reaction as a tool for investigating the molecular
epidemiology of visceral leishmaniasis by estimating the risk of
disease transmission in endemic areas, with the kDNA primer
representing the most reliable marker for the parasite.</abstract><cop>Brazil</cop><pub>Fundação Oswaldo Cruz, Fiocruz</pub><pmid>25004147</pmid><doi>10.1590/0074-0276130285</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Memórias do Instituto Oswaldo Cruz, 2014-07, Vol.109 (4), p.442-447 |
| issn | 1678-8060 0074-0276 1678-8060 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_01aaabb2e2104919a3a9e1ac433925ee |
| source | SciELO Brazil; PubMed Central Free |
| subjects | Animals DNA Primers - genetics DNA, Kinetoplast - genetics DNA, Protozoan - genetics Female Genetic Markers Insect Vectors - classification Insect Vectors - parasitology Leishmania (Leishmania) infantum Leishmania infantum - genetics Leishmania infantum - isolation & purification Lutzomyia longipalpis PARASITOLOGY Polymerase Chain Reaction Psychodidae - classification Psychodidae - parasitology Sensitivity and Specificity TROPICAL MEDICINE visceral leishmaniasis |
| title | A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum |
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