Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide

Staphylococcus aureus and coagulase-negative staphylococci (CoNS) have become the main causative agents of medical device-related infections due to their biofilm-forming capability, which protects them from the host’s immune system and from the action of antimicrobials. This study evaluated the abil...

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Bibliographic Details
Published in:Antibiotics (Basel) 2021-07, Vol.10 (7), p.879
Main Authors: de Oliveira, Adilson, Pinheiro-Hubinger, Luiza, Pereira, Valéria Cataneli, Riboli, Danilo Flávio Moraes, Martins, Katheryne Benini, Romero, Letícia Calixto, Cunha, Maria de Lourdes Ribeiro de Souza da
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
Bacteria
biofilm structure
Biofilms
catheter
Catheters
Coagulase
coagulase-negative staphylococci
Electron microscopy
Gene expression
Immune system
Infections
Medical equipment
Medical instruments
Peptides
Polymerase chain reaction
Polystyrene
Polystyrene resins
Population density
Proteins
quorum sensing
RIP
Scanning electron microscopy
Segments
Staphylococcus aureus
Staphylococcus infections
Virulence
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Summary:Staphylococcus aureus and coagulase-negative staphylococci (CoNS) have become the main causative agents of medical device-related infections due to their biofilm-forming capability, which protects them from the host’s immune system and from the action of antimicrobials. This study evaluated the ability of RNA III inhibiting peptide (RIP) to inhibit biofilm formation in 10 strains isolated from clinical materials, including one S. aureus strain, two S. epidermidis, two S. haemolyticus, two S. lugdunensis, and one isolate each of the following species: S. warneri, S. hominis, and S. saprophyticus. The isolates were selected from a total of 200 strains evaluated regarding phenotypic biofilm production and the presence and expression of the ica operon. The isolates were cultured in trypticase soy broth with 2% glucose in 96-well polystyrene plates containing catheter segments in the presence and absence of RIP. The catheter segments were observed by scanning electron microscopy. The results showed inhibition of biofilm formation in the presence of RIP in all CoNS isolates; however, RIP did not interfere with biofilm formation by S. aureus. RIP is a promising tool that might be used in the future for the prevention of biofilm-related infections caused by CoNS.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics10070879