Klebsiella pneumoniae employs a type VI secretion system to overcome microbiota-mediated colonization resistance

• Published in: Nature communications 2025-01, Vol.16 (1), p.940-17, Article 940
• Main Authors: Bray, Andrew S., Broberg, Christopher A., Hudson, Andrew W., Wu, Weisheng, Nagpal, Ravinder K., Islam, Maidul, Valencia-Bacca, Juan D., Shahid, Fawaz, Hernandez, Giovanna E., Nutter, Noah A., Walker, Kimberly A., Bennett, Emma F., Young, Taylor M., Barnes, Andrew J., Ornelles, David A., Miller, Virginia L., Zafar, M. Ammar
• Format: Article
• Language: English
• Subjects: 45/41; 45/70; 45/77; 45/88; 45/90; 49/47; 631/326/1320; 631/326/2565/2134; 631/326/421; 64/60; 82/83; Animal models; Animals; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Colonization; Digestive system; DNA Transposable Elements - genetics; Drug resistance; Female; Gastrointestinal Microbiome - genetics; Gastrointestinal tract; Gastrointestinal Tract - microbiology; Gene expression; Gene Expression Regulation, Bacterial; Gram-negative bacteria; Humanities and Social Sciences; Intestinal microflora; Klebsiella; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - metabolism; Metagenomics; Mice; Mice, Inbred C57BL; Microbiomes; Microbiota; Microorganisms; multidisciplinary; Mutagenesis; Nosocomial infection; Nutrients; Science; Science (multidisciplinary); Secretion; Transposon mutagenesis; Transposons; Type VI Secretion Systems - genetics; Type VI Secretion Systems - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-025-56309-8

Microbial species must compete for space and nutrients to persist in the gastrointestinal (GI) tract, and our understanding of the complex pathobiont-microbiota interactions is far from complete. Klebsiella pneumoniae , a problematic, often drug-resistant nosocomial pathogen, can colonize the GI tract asymptomatically, serving as an infection reservoir. To provide insight on how K. pneumoniae interacts with the resident gut microbiome, we conduct a transposon mutagenesis screen using a murine model of GI colonization with an intact microbiota. Among the genes identified were those encoding a type VI secretion system (T6SS), which mediates contact-dependent killing of gram-negative bacteria. From several approaches, we demonstrate that the T6SS is critical for K. pneumoniae gut colonization. Metagenomics and in vitro killing assays reveal that K. pneumoniae reduces Betaproteobacteria species in a T6SS-dependent manner, thus identifying specific species targeted by K. pneumoniae . We further show that T6SS gene expression is controlled by several transcriptional regulators and that expression only occurs in vitro under conditions that mimic the gut environment. By enabling K. pneumoniae to thrive in the gut, the T6SS indirectly contributes to the pathogenic potential of this organism. These observations advance our molecular understanding of how K. pneumoniae successfully colonizes the GI tract. Through metagenomics, Bray et al. identified Klebsiella pneumoniae interacting microbiota partners in the gut, and a transposon mutagenesis screen revealed a role for its T6SS in gut colonization, with t6ss transcription only occurring in vitro under GI-mimetic conditions.