Exogenous ACC Deaminase Is Key to Improving the Performance of Pasture Legume-Rhizobial Symbioses in the Presence of a High Manganese Concentration

Manganese (Mn) toxicity is a very common soil stress around the world, which is responsible for low soil fertility. This manuscript evaluates the effect of the endophytic bacterium sp. Q1 on different rhizobial-legume symbioses in the absence and presence of Mn toxicity. Three legume species, (chick...

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Bibliographic Details
Published in:Plants (Basel) 2020-11, Vol.9 (12), p.1630
Main Authors: Paço, Ana, da-Silva, José Rodrigo, Torres, Denise Pereira, Glick, Bernard R, Brígido, Clarisse
Format: Article
Language:English
Subjects:
abiotic stress
Agricultural production
Alfalfa
Bacteria
Binding sites
Chickpeas
Endophytes
Enzymes
Gene disruption
Genomes
Legumes
Manganese
Mutants
nodulation
Pasture
Plant growth
plant growth promotion
plant growth-promoting traits
plant–bacteria interactions
Proteins
Soil conditions
Soil fertility
Soil stresses
Soils
Symbiosis
Toxicity
Trifolium subterraneum
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Summary:Manganese (Mn) toxicity is a very common soil stress around the world, which is responsible for low soil fertility. This manuscript evaluates the effect of the endophytic bacterium sp. Q1 on different rhizobial-legume symbioses in the absence and presence of Mn toxicity. Three legume species, (chickpea), (subterranean clover), and (burr medic) were used. To evaluate the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase produced by strain Q1 in these interactions, an ACC deaminase knockout mutant of this strain was constructed and used in those trials. The Q1 strain only promoted the symbiotic performance of bv. trifolii ATCC 14480 and ATCC 9930 , leading to an increase of the growth of their hosts in both conditions. Notably, the gene disruption of strain Q1 abolished the beneficial effect of this bacterium as well as causing this mutant strain to act deleteriously in those specific symbioses. This study suggests that the addition of non-rhizobia with functional ACC deaminase may be a strategy to improve the pasture legume-rhizobial symbioses, particularly when the use of rhizobial strains alone does not yield the expected results due to their difficulty in competing with native strains or in adapting to inhibitory soil conditions.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants9121630