Exploring Honeybee Abdominal Anatomy through Micro-CT and Novel Multi-Staining Approaches

Continuous improvements in morphological and histochemical analyses of Apis mellifera could improve our understanding of the anatomy and physiology of these insects at both the cellular and tissue level. In this work, two different approaches have been performed to add new data on the abdomen of wor...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2022-06, Vol.13 (6), p.556
Main Authors: De Paula, Jessica Carreira, Doello, Kevin, Mesas, Cristina, Kapravelou, Garyfalia, Cornet-Gómez, Alberto, Orantes, Francisco José, Martínez, Rosario, Linares, Fátima, Prados, Jose Carlos, Porres, Jesus María, Osuna, Antonio, de Pablos, Luis Miguel
Format: Article
Language:English
Subjects:
Abdomen
Acids
Anatomy
Apis mellifera
bee
Bees
Biodiversity
Computed tomography
Drinking water
Environmental conditions
Epithelium
Ethanol
Ganglia
Hindgut
Hymenoptera
Image resolution
Insects
Micro-CT
Micrometers
Microscopy
Midgut
Physiology
Plant reproduction
Pollination
Pollinators
stain
Staining
Stains & staining
Ventricle
X-rays
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Summary:Continuous improvements in morphological and histochemical analyses of Apis mellifera could improve our understanding of the anatomy and physiology of these insects at both the cellular and tissue level. In this work, two different approaches have been performed to add new data on the abdomen of worker bees: (i) Micro-computed tomography (Micro-CT), which allows the identification of small-scale structures (micrometers) with adequate/optimal resolution and avoids sample damage and, (ii) histochemical multi-staining with Periodic Acid-Schiff-Alcian blue, Lactophenol-Saphranin O and pentachrome staining to precisely characterize the histological structures of the midgut and hindgut. Micro-CT allowed high-resolution imaging of anatomical structures of the honeybee abdomen with particular emphasis on the proventriculus and pyloric valves, as well as the connection of the sting apparatus with the terminal abdominal ganglia. Furthermore, the histochemical analyses have allowed for the first-time description of ventricular telocytes in honeybees, a cell type located underneath the midgut epithelium characterized by thin and long cytoplasmic projections called telopodes. Overall, the analysis of these images could help the detailed anatomical description of the cryptic structures of honeybees and also the characterization of changes due to abiotic or biotic stress conditions.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects13060556