Determination of prilocaine HCl in bulk drug and pharmaceutical formulation by GC-NPD method

The novel analytical method was developed and validated for determination of prilocaine HCl in bulk drug and pharmaceutical formulation by gas chromatography-nitrogen phosphorus detection (GC-NPD). The chromatographic separation was performed using a HP-5MS column. The calibration curve was linear o...

Full description

Saved in:
Bibliographic Details
Published in:Chemical Industry and Chemical Engineering Quarterly 2013-01, Vol.19 (2), p.313-319
Main Authors: Atila, Alptug, Kadioglu, Yucel
Format: Article
Language:English
Subjects:
GC-NPD
pharmaceutical formulation
prilocaine HCl
validation
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The novel analytical method was developed and validated for determination of prilocaine HCl in bulk drug and pharmaceutical formulation by gas chromatography-nitrogen phosphorus detection (GC-NPD). The chromatographic separation was performed using a HP-5MS column. The calibration curve was linear over the concentration range of 40-1000 ng ml-1 with a correlation coefficient of 0.9998. The limits of detection (LOD) and quantification (LOQ) of method were 10 ng ml-1 and 35 ng ml-1, respectively. The within-day and between-day precision, expressed as the percent relative standard deviation (RSD%) was less than 5.0%, and accuracy (percent relative error) was better than 4.0%. The developed method can be directly and easily applied for determination of prilocaine HCl in bulk drug and pharmaceutical formulation using internal standard methodology. nema
ISSN:1451-9372
2217-7434
DOI:10.2298/CICEQ120124064A