Damage-free light-induced assembly of intestinal bacteria with a bubble-mimetic substrate

Rapid evaluation of functions in densely assembled bacteria is a crucial issue in the efficient study of symbiotic mechanisms. If the interaction between many living microbes can be controlled and accelerated via remote assembly, a cultivation process requiring a few days can be ommitted, thus leadi...

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Bibliographic Details
Published in:Communications biology 2021-03, Vol.4 (1), p.385-385, Article 385
Main Authors: Hayashi, Kota, Yamamoto, Yasuyuki, Tamura, Mamoru, Tokonami, Shiho, Iida, Takuya
Format: Article
Language:English
Subjects:
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Bacteria
Biology
Biomedical and Life Sciences
Biomimetic Materials - chemistry
Gastrointestinal Microbiome - radiation effects
Intestine
Intestines - microbiology
Lactic acid
Lactic acid bacteria
Lacticaseibacillus casei - radiation effects
Lasers
Life Sciences
Light effects
Microbial Viability
Polystyrenes - chemistry
Quorum Sensing - radiation effects
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Summary:Rapid evaluation of functions in densely assembled bacteria is a crucial issue in the efficient study of symbiotic mechanisms. If the interaction between many living microbes can be controlled and accelerated via remote assembly, a cultivation process requiring a few days can be ommitted, thus leading to a reduction in the time needed to analyze the bacterial functions. Here, we show the rapid, damage-free, and extremely dense light-induced assembly of microbes over a submillimeter area with the “bubble-mimetic substrate (BMS)”. In particular, we successfully assembled 10 4 –10 5 cells of lactic acid bacteria ( Lactobacillus casei ), achieving a survival rate higher than 95% within a few minutes without cultivation process. This type of light-induced assembly on substrates like BMS, with the maintenance of the inherent functions of various biological samples, can pave the way for the development of innovative methods for rapid and highly efficient analysis of functions in a variety of microbes. Hayashi et al. propose a method to realise rapid, damage-free, remote light-induced assembly of microbes over a submillimeter area with the “bubble-mimetic substrate”. They assemble 10 4 –10 5 cells of lactic acid bacteria with a survival rate higher than 95% around a laser spot. This technique is promising in developing methods for rapid and high-efficiency functional analysis of microbes.
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-021-01807-w