Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis

Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important implications i...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2011-10, Vol.44 (5), p.572-575
Main Authors: Coutinho, Carlos Eduardo Ribeiro, Santos, Dilvani Oliveira, Baptista, Cibele, Figueiredo, Fabiano Borges, Madeira, Maria de Fatima
Format: Article
Language:English
Subjects:
Animals
Brazil
Cão
Dog Diseases - parasitology
Dogs
Domestic animals
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Genetic Variation - genetics
Leishmania chagasi
Leishmania infantum - genetics
Leishmania infantum - isolation & purification
Leishmaniasis, Visceral - parasitology
Leishmaniasis, Visceral - veterinary
MLEE
Parasites
Phenotype
Subpopulations
TROPICAL MEDICINE
Variabilidade genética
Vector-borne diseases
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Summary:Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important implications in the clinical-epidemiological context. The objective of this study was to evaluate and compare the phenotypic variability of 153 L. chagasi strains isolated from dogs originating from the municipalities of Rio de Janeiro (n = 57) and Belo Horizonte (n = 96), where the disease is endemic. Strains isolated only from intact skin were selected and analyzed by multilocus enzyme electrophoresis using nine enzyme systems (6PG, GPI, NH1 and NH2, G6P, PGM, MDH, ME, and IDHNADP). The electrophoretic profile was identical for all isolates analyzed and was the same as that of the L. chagasi reference strain (MHOM/BR/74/PP75). Phenetic analysis showed a similarity index of one for all strains, with the isolates sharing 100% of the characteristics analyzed. The results demonstrate that the L. chagasi populations circulating in dogs from Rio de Janeiro and Belo Horizonte belong to a single zymodeme.
ISSN:0037-8682
1678-9849
1678-9849
DOI:10.1590/S0037-86822011005000049