A High-Density Integrated DArTseq SNP-Based Genetic Map of Pisum fulvum and Identification of QTLs Controlling Rust Resistance

, a wild relative of pea is an important source of allelic diversity to improve the genetic resistance of cultivated species against fungal diseases of economic importance like the pea rust caused by . To unravel the genetic control underlying resistance to this fungal disease, a recombinant inbred...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in plant science 2018-02, Vol.9, p.167-167
Main Authors: Barilli, Eleonora, Cobos, María J, Carrillo, Estefanía, Kilian, Andrzej, Carling, Jason, Rubiales, Diego
Format: Article
Language:English
Subjects:
DArT
genetic linkage map
Life Sciences
Pisum fulvum
Plant Science
QTL
rust resistance
Uromyces pisi
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:, a wild relative of pea is an important source of allelic diversity to improve the genetic resistance of cultivated species against fungal diseases of economic importance like the pea rust caused by . To unravel the genetic control underlying resistance to this fungal disease, a recombinant inbred line (RIL) population was generated from a cross between two accessions, IFPI3260 and IFPI3251, and genotyped using Diversity Arrays Technology. A total of 9,569 high-quality DArT-Seq and 8,514 SNPs markers were generated. Finally, a total of 12,058 markers were assembled into seven linkage groups, equivalent to the number of haploid chromosomes of and . The newly constructed integrated genetic linkage map of covered an accumulated distance of 1,877.45 cM, an average density of 1.19 markers cM and an average distance between adjacent markers of 1.85 cM. The composite interval mapping revealed three QTLs distributed over two linkage groups that were associated with the percentage of rust disease severity (DS%). QTLs and were located in the LGs II and IV respectively and were consistently identified both in adult plants over 3 years at the field (Córdoba, Spain) and in seedling plants under controlled conditions. Whenever they were detected, their contribution to the total phenotypic variance varied between 19.8 and 29.2. A third QTL ( ) was also located in the LGIVand was environmentally specific as was only detected for DS % in seedlings under controlled conditions. It accounted more than 14% of the phenotypic variation studied. Taking together the data obtained in the study, it could be concluded that the expression of resistance to fungal diseases in originates from the resistant parent IFPI3260.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2018.00167