Genome-wide identification of carbapenem-resistant Gram-negative bacterial (CR-GNB) isolates retrieved from hospitalized patients in Bihar, India

• Published in: Scientific reports 2022-05, Vol.12 (1), p.8477-8477, Article 8477
• Main Authors: Kumari, Namrata, Kumar, Mukesh, Katiyar, Amit, Kumar, Abhay, Priya, Pallavi, Kumar, Bablu, Biswas, Nihar Ranjan, Kaur, Punit
• Format: Article
• Language: English
• Subjects: 631/114/2163; 631/114/2785; 631/326/22; 631/326/41; 631/326/421; Antibiotics; Carbapenemase; Clinical isolates; Developing countries; Drug resistance; E coli; Genomes; Genomic analysis; Gram-negative bacteria; Humanities and Social Sciences; LDCs; multidisciplinary; Patients; Public health; Science; Science (multidisciplinary); Whole genome sequencing; β Lactamase; β-Lactam antibiotics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-12471-3

Carbapenemase-producing clinical isolates are becoming more common over the world, posing a severe public health danger, particularly in developing nations like India. Carbapenem-resistant Gram-negative bacterial (CR-GNB) infection has become a fast-expanding global threat with limited antibiotic choice and significant mortality. This study aimed to highlight the carbapenem-resistance among clinical isolates of hospital admitted patients in Bihar, India. A cross-sectional study was conducted with 101 clinical isolates of Escherichia coli , Klebsiella pneumoniae , Acinetobacter baumannii , and Pseudomonas aeruginosa. All GNB isolates were tested for their antimicrobial susceptibility using Kirby-Bauer disc diffusion method. Double disc synergy test / modified Hodge test (DDST/MHT) were used to detect carbapenemase production by these isolates. Subsequently, these isolates were evaluated for carbapenem-resistance genes using whole-genome sequencing method. The overall percentage of carbapenem-resistance among GNB was (17/101) 16.8%. The genomic analysis of antimicrobial-resistance (AMR) demonstrates a significantly high prevalence of bla CTX-M followed by bla SHV , bla TEM , bla OXA, and bla NDM β-lactam or carbapenem resistance genes among clinical isolates of GNB. Co-occurrence of bla NDM with other beta-lactamase-encoding genes was found in 70.6% of carbapenemase-producing isolates. Our study highlights the mechanism of carbapenem-resistance to curb the overwhelming threat posed by the emergence of drug-resistance in India.