Covalent IR820-PEG-diamine nanoconjugates for theranostic applications in cancer

Near-infrared dyes can be used as theranostic agents in cancer management, based on their optical imaging and localized hyperthermia capabilities. However, their clinical translatability is limited by issues such as photobleaching, short circulation times, and nonspecific biodistribution. Nanoconjug...

Full description

Saved in:
Bibliographic Details
Published in:International journal of nanomedicine 2014-01, Vol.9 (Issue 1), p.4631-4648
Main Authors: Fernandez-Fernandez, Alicia, Manchanda, Romila, Carvajal, Denny A, Lei, Tingjun, Srinivasan, Supriya, McGoron, Anthony J
Format: Article
Language:English
Subjects:
Algorithms
Animals
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacokinetics
Cancer
Cancer therapies
Care and treatment
Cell Line, Tumor
Cell Proliferation - drug effects
Chemotherapy
Diamines - chemistry
Diamines - toxicity
Drug delivery systems
Drugs
Dyes
Fever
fluorescent imaging
Humans
Hyperthermia
Hyperthermia, Induced
image-guided therapy
Indocyanine Green - analogs & derivatives
Indocyanine Green - chemistry
Indocyanine Green - pharmacokinetics
Indocyanine Green - toxicity
Mice
Nanoconjugates - chemistry
Nanoconjugates - toxicity
Nanoparticles
Nanotechnology
NMR
Nuclear magnetic resonance
Optical Imaging - methods
Original Research
Physiological aspects
Plasma
Polyethylene glycol
Polyethylene Glycols - chemistry
Polyethylene Glycols - toxicity
Properties
Surgery, Computer-Assisted
Tissue Distribution
Vehicles
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Near-infrared dyes can be used as theranostic agents in cancer management, based on their optical imaging and localized hyperthermia capabilities. However, their clinical translatability is limited by issues such as photobleaching, short circulation times, and nonspecific biodistribution. Nanoconjugate formulations of cyanine dyes, such as IR820, may be able to overcome some of these limitations. We covalently conjugated IR820 with 6 kDa polyethylene glycol (PEG)-diamine to create a nanoconjugate (IRPDcov) with potential for in vivo applications. The conjugation process resulted in nearly spherical, uniformly distributed nanoparticles of approximately 150 nm diameter and zeta potential -0.4±0.3 mV. The IRPDcov formulation retained the ability to fluoresce and to cause hyperthermia-mediated cell-growth inhibition, with enhanced internalization and significantly enhanced cytotoxic hyperthermia effects in cancer cells compared with free dye. Additionally, IRPDcov demonstrated a significantly longer (P
ISSN:1178-2013
1176-9114
1178-2013
DOI:10.2147/IJN.S69550