Introgression of SbERD4 Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic Tobacco

• Published in: Cells (Basel, Switzerland) Switzerland), 2021-12, Vol.11 (1), p.62
• Main Authors: Jha, Rajesh Kumar, Mishra, Avinash
• Format: Article
• Language: English
• Subjects: Abiotic stress; Adaptation, Physiological - genetics; Antioxidants; Chenopodiaceae - genetics; Climate change; Cloning; Cold; Dehydration; Drought; Electrolyte leakage; Food security; Gene expression; Gene Expression Regulation, Plant; Genetic engineering; genetic transformation; halophyte; Halophytes; Heat-Shock Proteins - genetics; Homeostasis; Hydrogen peroxide; Ions; Localization; Membrane proteins; Membrane Proteins - metabolism; Nicotiana - genetics; Nicotiana - physiology; Osmosis; Osmotic stress; Physiology; Plant Development - genetics; Plant Proteins - genetics; Plants, Genetically Modified; Polyphenols; Proline; Proteins; Reactive Oxygen Species - metabolism; Salinity; Salinity effects; Signal transduction; Stress, Physiological - genetics; Subcellular Fractions - metabolism; Tobacco; Transcription; Transformation, Genetic; transgenic; Transgenic plants
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells11010062

is an extreme halophyte that commonly grows on marsh conditions and is also considered a promising resource for drought and salt-responsive genes. To unveil a glimpse of stress endurance by plants, it is of the utmost importance to develop an understanding of stress tolerance mechanisms. 'Early Responsive to Dehydration' (ERD) genes are defined as a group of genes involved in stress tolerance and the development of plants. To increase this understanding, parallel to this expedited thought, a novel gene was cloned from , characterized, and functionally validated in the model plant tobacco. The study showed that is a plasma-membrane bound protein, and its overexpression in tobacco plants improved salinity and osmotic stress tolerance. Transgenic plants showed high relative water, chlorophylls, sugars, starch, polyphenols, proline, free amino acids, and low electrolyte leakage and H O content compared to control plants (wild type and vector control) under different abiotic stress conditions. Furthermore, the transcript expression of antioxidant enzyme encoding genes , , , and showed higher expression in transgenic compared to wild-type and vector controls under varying stress conditions. Overall, the overexpression of a novel early responsive to dehydration stress protein 4-encoding gene ( ) enhanced the tolerance of the plant against multiple abiotic stresses. In conclusion, the overexpression of the gene mitigates plant physiology by enduring stress tolerance and might be considered as a promising key gene for engineering salinity and drought stress tolerance in crops.