Strain Characterisation for Measuring Bioefficacy of ITNs Treated with Two Active Ingredients (Dual-AI ITNs): Developing a Robust Protocol by Building Consensus

Durability monitoring of insecticide-treated nets (ITNs) containing a pyrethroid in combination with a second active ingredient (AI) must be adapted so that the insecticidal bioefficacy of each AI can be monitored independently. An effective way to do this is to measure rapid knock down of a pyrethr...

Full description

Saved in:
Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2022-05, Vol.13 (5), p.434
Main Authors: Lees, Rosemary S, Armistead, Jennifer S, Azizi, Salum, Constant, Edi, Fornadel, Christen, Gimnig, John E, Hemingway, Janet, Impoinvil, Daniel, Irish, Seth R, Kisinza, William, Lissenden, Natalie, Mawejje, Henry D, Messenger, Louisa A, Moore, Sarah, Ngufor, Corine, Oxborough, Richard, Protopopoff, Natacha, Ranson, Hilary, Small, Graham, Wagman, Joseph, Weetman, David, Zohdy, Sarah, Spiers, Angus
Format: Article
Language:English
Subjects:
Bioassays
Bioavailability
Control
Culicidae
Durability
Enzymes
Guidelines
Insecticide resistance
insecticide-treated nets (ITN)
Insecticides
Laboratories
Life span
Malaria
Metabolism
method development
Monitoring
Mortality
mosquito
Mosquitoes
Nets
Product development
pyrethroid
Pyrethroids
Robustness
strain characterisation
Test facilities
Vector-borne diseases
Wear resistance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Durability monitoring of insecticide-treated nets (ITNs) containing a pyrethroid in combination with a second active ingredient (AI) must be adapted so that the insecticidal bioefficacy of each AI can be monitored independently. An effective way to do this is to measure rapid knock down of a pyrethroid-susceptible strain of mosquitoes to assess the bioefficacy of the pyrethroid component and to use a pyrethroid-resistant strain to measure the bioefficacy of the second ingredient. To allow robust comparison of results across tests within and between test facilities, and over time, protocols for bioefficacy testing must include either characterisation of the resistant strain, standardisation of the mosquitoes used for bioassays, or a combination of the two. Through a series of virtual meetings, key stakeholders and practitioners explored different approaches to achieving these goals. Via an iterative process we decided on the preferred approach and produced a protocol consisting of characterising mosquitoes used for bioefficacy testing before and after a round of bioassays, for example at each time point in a durability monitoring study. We present the final protocol and justify our approach to establishing a standard methodology for durability monitoring of ITNs containing pyrethroid and a second AI.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects13050434