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Detection of white sturgeon iridovirus (WSIV) in wild sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) in Poland
White sturgeon iridovirus (WSIV) disease is caused by a virus of the eponymous family and is mostly triggered by stressful environmental conditions, . high rearing density, excessive handling, or temporary loss of water. The aim of this study was to develop the most effective diagnostic method for q...
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| Published in: | Journal of veterinary research 2020-09, Vol.64 (3), p.363-368 |
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| creator | Hofsoe-Oppermann, Paulina Kiełpińska, Jolanta Panicz, Remigiusz Bergmann, Sven M. |
| description | White sturgeon iridovirus (WSIV) disease is caused by a virus of the eponymous family and is mostly triggered by stressful environmental conditions,
. high rearing density, excessive handling, or temporary loss of water. The aim of this study was to develop the most effective diagnostic method for quick and efficient confirmation or exclusion of the presence of WSIV.
A total of 42 samples (spleen, gills, intestine, skin, kidney, and brain) were collected from eight sturgeon (
and
) aged ≤5+ farmed or caught between 2010 and 2014 in open waters (Dąbie Lake and Szczecin Lagoon). They were tested for WSIV presence using conventional PCR, qPCR, and
hybridisation (ISH).
In gross examination, all fish appeared to be healthy. Neither species showed clinical signs typical of WSIV infection. In the majority of cases, fragments of iridoviral DNA were found using molecular methods in the kidneys, and also in the liver, gills, and skin. The detection rate using ISH was 47.37% and most commonly the brain and kidney tissues were positive. The most efficient of the methods used was real-time PCR, with 100% effectiveness in detection of WSIV DNA.
The study demonstrates the capabilities for WSIV diagnosis available to sturgeon farmers and water administrators, indicating useful methods of adequate sensitivity as well as organs to sample in order to achieve the highest probability of viral detection. |
| doi_str_mv | 10.2478/jvetres-2020-0055 |
| format | article |
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. high rearing density, excessive handling, or temporary loss of water. The aim of this study was to develop the most effective diagnostic method for quick and efficient confirmation or exclusion of the presence of WSIV.
A total of 42 samples (spleen, gills, intestine, skin, kidney, and brain) were collected from eight sturgeon (
and
) aged ≤5+ farmed or caught between 2010 and 2014 in open waters (Dąbie Lake and Szczecin Lagoon). They were tested for WSIV presence using conventional PCR, qPCR, and
hybridisation (ISH).
In gross examination, all fish appeared to be healthy. Neither species showed clinical signs typical of WSIV infection. In the majority of cases, fragments of iridoviral DNA were found using molecular methods in the kidneys, and also in the liver, gills, and skin. The detection rate using ISH was 47.37% and most commonly the brain and kidney tissues were positive. The most efficient of the methods used was real-time PCR, with 100% effectiveness in detection of WSIV DNA.
The study demonstrates the capabilities for WSIV diagnosis available to sturgeon farmers and water administrators, indicating useful methods of adequate sensitivity as well as organs to sample in order to achieve the highest probability of viral detection.</description><identifier>ISSN: 2450-7393</identifier><identifier>ISSN: 2450-8608</identifier><identifier>EISSN: 2450-8608</identifier><identifier>DOI: 10.2478/jvetres-2020-0055</identifier><identifier>PMID: 32984624</identifier><language>eng</language><publisher>Poland: Sciendo</publisher><subject>acipenser gueldenstaedtii ; acipenser oxyrinchus ; Deoxyribonucleic acid ; disease control ; DNA ; Environmental conditions ; freshwater aquaculture ; Gills ; Hybridization ; Intestine ; Kidneys ; Lagoons ; Polymerase chain reaction ; Spleen ; Sturgeon ; vectors</subject><ispartof>Journal of veterinary research, 2020-09, Vol.64 (3), p.363-368</ispartof><rights>2020 P. Hofsoe-Oppermann et al. published by Sciendo.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by-nc-nd/3.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 P. Hofsoe-Oppermann et al. published by Sciendo 2020 P. Hofsoe-Oppermann et al. published by Sciendo</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-2be4e1b4fc6e8b222ac350b747f2046943fa9e86f88880a70db4b0cff3e7daa13</citedby><cites>FETCH-LOGICAL-c549t-2be4e1b4fc6e8b222ac350b747f2046943fa9e86f88880a70db4b0cff3e7daa13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497756/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2544440903?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32984624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hofsoe-Oppermann, Paulina</creatorcontrib><creatorcontrib>Kiełpińska, Jolanta</creatorcontrib><creatorcontrib>Panicz, Remigiusz</creatorcontrib><creatorcontrib>Bergmann, Sven M.</creatorcontrib><title>Detection of white sturgeon iridovirus (WSIV) in wild sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) in Poland</title><title>Journal of veterinary research</title><addtitle>J Vet Res</addtitle><description>White sturgeon iridovirus (WSIV) disease is caused by a virus of the eponymous family and is mostly triggered by stressful environmental conditions,
. high rearing density, excessive handling, or temporary loss of water. The aim of this study was to develop the most effective diagnostic method for quick and efficient confirmation or exclusion of the presence of WSIV.
A total of 42 samples (spleen, gills, intestine, skin, kidney, and brain) were collected from eight sturgeon (
and
) aged ≤5+ farmed or caught between 2010 and 2014 in open waters (Dąbie Lake and Szczecin Lagoon). They were tested for WSIV presence using conventional PCR, qPCR, and
hybridisation (ISH).
In gross examination, all fish appeared to be healthy. Neither species showed clinical signs typical of WSIV infection. In the majority of cases, fragments of iridoviral DNA were found using molecular methods in the kidneys, and also in the liver, gills, and skin. The detection rate using ISH was 47.37% and most commonly the brain and kidney tissues were positive. The most efficient of the methods used was real-time PCR, with 100% effectiveness in detection of WSIV DNA.
The study demonstrates the capabilities for WSIV diagnosis available to sturgeon farmers and water administrators, indicating useful methods of adequate sensitivity as well as organs to sample in order to achieve the highest probability of viral detection.</description><subject>acipenser gueldenstaedtii</subject><subject>acipenser oxyrinchus</subject><subject>Deoxyribonucleic acid</subject><subject>disease control</subject><subject>DNA</subject><subject>Environmental conditions</subject><subject>freshwater aquaculture</subject><subject>Gills</subject><subject>Hybridization</subject><subject>Intestine</subject><subject>Kidneys</subject><subject>Lagoons</subject><subject>Polymerase chain reaction</subject><subject>Spleen</subject><subject>Sturgeon</subject><subject>vectors</subject><issn>2450-7393</issn><issn>2450-8608</issn><issn>2450-8608</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp1kk1v1DAQhiMEolXpD-CCInEph8DEH3HCAbQqXytVAomvo-XY49SrbLy1k12t-PN4u8vSIjEXj2beeWSP3yx7WsJLwkT9arHGMWAsCBAoADh_kJ0SxqGoK6gfHnJBG3qSnce4AIBSUNGU9HF2QklTs4qw0-zXOxxRj84Pubf55tqNmMdxCh2migvO-LULU8wvfn6d_3iRuyHfuN4cJakxS9ODX40Ytp1zr_OZdiscIgZnfVhivFsxCm8ZX3yvBvMke2RVH_H8cJ5l3z-8_3b5qbj6_HF-ObsqNGfNWJAWGZYts7rCuiWEKE05tIIJS4BVDaNWNVhXtk4BSoBpWQvaWorCKFXSs2y-5xqvFnIV3FKFrfTKyduCD51UYXS6Rwmt5pxRIywtmQXeNonIWWkqpiuiIbHe7FmrqV2i0TiMQfX3oPc7g7uWnV9LwRoheJUAFwdA8DcTxlEuXdTYp4Wgn6IkLD2pKUnNk_T5P9KFn8KQViUJZymgAZpU5V6lg48xoD1epgS5c4o8OEXunCJ3Tkkzz-6-4jjxxxdJ8HYv2Kg-_azBLkzblPy9wX_hFaO0ovQ3Q2nSQA</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Hofsoe-Oppermann, Paulina</creator><creator>Kiełpińska, Jolanta</creator><creator>Panicz, Remigiusz</creator><creator>Bergmann, Sven M.</creator><general>Sciendo</general><general>De Gruyter Poland</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20200901</creationdate><title>Detection of white sturgeon iridovirus (WSIV) in wild sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) in Poland</title><author>Hofsoe-Oppermann, Paulina ; Kiełpińska, Jolanta ; Panicz, Remigiusz ; Bergmann, Sven M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-2be4e1b4fc6e8b222ac350b747f2046943fa9e86f88880a70db4b0cff3e7daa13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>acipenser gueldenstaedtii</topic><topic>acipenser oxyrinchus</topic><topic>Deoxyribonucleic acid</topic><topic>disease control</topic><topic>DNA</topic><topic>Environmental conditions</topic><topic>freshwater aquaculture</topic><topic>Gills</topic><topic>Hybridization</topic><topic>Intestine</topic><topic>Kidneys</topic><topic>Lagoons</topic><topic>Polymerase chain reaction</topic><topic>Spleen</topic><topic>Sturgeon</topic><topic>vectors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hofsoe-Oppermann, Paulina</creatorcontrib><creatorcontrib>Kiełpińska, Jolanta</creatorcontrib><creatorcontrib>Panicz, Remigiusz</creatorcontrib><creatorcontrib>Bergmann, Sven M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hofsoe-Oppermann, Paulina</au><au>Kiełpińska, Jolanta</au><au>Panicz, Remigiusz</au><au>Bergmann, Sven M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of white sturgeon iridovirus (WSIV) in wild sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) in Poland</atitle><jtitle>Journal of veterinary research</jtitle><addtitle>J Vet Res</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>64</volume><issue>3</issue><spage>363</spage><epage>368</epage><pages>363-368</pages><issn>2450-7393</issn><issn>2450-8608</issn><eissn>2450-8608</eissn><abstract>White sturgeon iridovirus (WSIV) disease is caused by a virus of the eponymous family and is mostly triggered by stressful environmental conditions,
. high rearing density, excessive handling, or temporary loss of water. The aim of this study was to develop the most effective diagnostic method for quick and efficient confirmation or exclusion of the presence of WSIV.
A total of 42 samples (spleen, gills, intestine, skin, kidney, and brain) were collected from eight sturgeon (
and
) aged ≤5+ farmed or caught between 2010 and 2014 in open waters (Dąbie Lake and Szczecin Lagoon). They were tested for WSIV presence using conventional PCR, qPCR, and
hybridisation (ISH).
In gross examination, all fish appeared to be healthy. Neither species showed clinical signs typical of WSIV infection. In the majority of cases, fragments of iridoviral DNA were found using molecular methods in the kidneys, and also in the liver, gills, and skin. The detection rate using ISH was 47.37% and most commonly the brain and kidney tissues were positive. The most efficient of the methods used was real-time PCR, with 100% effectiveness in detection of WSIV DNA.
The study demonstrates the capabilities for WSIV diagnosis available to sturgeon farmers and water administrators, indicating useful methods of adequate sensitivity as well as organs to sample in order to achieve the highest probability of viral detection.</abstract><cop>Poland</cop><pub>Sciendo</pub><pmid>32984624</pmid><doi>10.2478/jvetres-2020-0055</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Publicly Available Content Database; PubMed Central |
| subjects | acipenser gueldenstaedtii acipenser oxyrinchus Deoxyribonucleic acid disease control DNA Environmental conditions freshwater aquaculture Gills Hybridization Intestine Kidneys Lagoons Polymerase chain reaction Spleen Sturgeon vectors |
| title | Detection of white sturgeon iridovirus (WSIV) in wild sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) in Poland |
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