Rapid Isolation of Low-Level Carbapenem-Resistant E. coli from Water and Foods Using Glycan-Coated Magnetic Nanoparticles

• Published in: Biosensors (Basel) 2023-09, Vol.13 (10), p.902
• Main Authors: Caliskan-Aydogan, Oznur, Sharief, Saad Asadullah, Alocilja, Evangelyn C.
• Format: Article
• Language: English
• Subjects: Antibiotics; Antibodies; Antimicrobial agents; Bacteria; Biological properties; Biological samples; Biosensors; Buffer solutions; Carbapenemase; carbapenemase-producing E. coli; Carbohydrates; Centrifugation; E coli; Electrophoresis; Food; Foodborne pathogens; Genes; Glycan; glycan-coated magnetic nanoparticles; Immunomagnetic separation; Infections; Inoculation; Klebsiella; Ligands; Low concentrations; magnetic extraction; Metallo-β-lactamase; Metallography; Microflora; Microscopy; Nanoparticles; Pathogens; Separation; Strains (organisms); Transmission electron microscopy
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios13100902

Carbapenem-resistant Enterobacterales (CRE) are one of the major global issues needing attention. Among them, carbapenemase-producing (CP) E. coli strains are commonly found in clinical and biological samples. Rapid and cost-effective detection of such strains is critical in minimizing their deleterious impact. While promising progress is being made in rapid detection platforms, separation and enrichment of bacteria are required to ensure the detection of low bacterial counts. The current separation methods, such as centrifugation, filtration, electrophoresis, and immunomagnetic separation, are often tedious, expensive, or ineffective for clinical and biological samples. Further, the extraction and concentration of antimicrobial-resistant bacteria (ARB) are not well documented. Thus, this study assessed the applicability of cost-effective glycan-coated magnetic nanoparticles (gMNPs) for simple and rapid extraction of CP E. coli. The study included two resistant (R)strains: Klebsiella pneumoniae carbapenemase (KPC)-producing E. coli (R: KPC) and New Delhi metallo-β-lactamase (NDM)-producing E. coli (R: NDM). A susceptible E. coli (S) strain was used as a control, a reference bacterium. The gMNPs successfully extracted and concentrated E. coli (R) and E. coli (S) at low concentrations from large volumes of buffer solution, water, and food samples. The gMNPs concentrated up to two and five times their initial concentration for E. coli (R) and E. coli (S) in the buffer solution, respectively. In water and food samples, the concentration of E. coli (S) and E. coli (R) were similar and ranged 1–3 times their initial inoculation. A variation in the concentration from different food samples was seen, displaying the impact of food microstructure and natural microflora. The cost-effective and rapid bacterial cell capture by gMNPs was achieved in 15 min, and its successful binding to the bacterial cells in the buffer solution and food matrices was also confirmed using Transmission Electron Microscopy (TEM). These results show promising applications of gMNPs to extract pathogens and ARB from biological samples.