Study of CaDreb2c and CaDreb2h Gene Sequences and Expression in Chickpea ( Cicer arietinum L.) Cultivars Growing in Northern Kazakhstan under Drought

• Published in: Plants (Basel) 2024-08, Vol.13 (15), p.2066
• Main Authors: Kiselev, Konstantin V, Ogneva, Zlata V, Dubrovina, Alexandra S, Gabdola, Ademi Zh, Khassanova, Gulmira Zh, Jatayev, Satyvaldy A
• Format: Article
• Language: English
• Subjects: abiotic stress; Agricultural production; Amino acids; Arid regions; Arid zones; Beans; Chickpeas; crop; Crop diseases; Cultivars; Dehydration; DNA sequencing; Drought; Drought resistance; Gene expression; Gene sequencing; Genes; Genetic research; Genetically altered foods; Greenhouse gases; Heat; Legumes; Mimosaceae; Molecular interactions; Nucleotide sequence; Nucleotides; Plant growth; Potatoes; Proteins; Rankings; Regulatory sequences; Salinity; Salinity tolerance; Substitutes; tolerance; Transcription factors; Water deprivation
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13152066

Drought poses a significant challenge to plant growth and productivity, particularly in arid regions like northern Kazakhstan. Dehydration-responsive element-binding (DREB) transcription factors play an important role in plant response to drought and other abiotic stresses. In , the DREB subfamily consists of six groups, designated DREB1 to DREB6. Among these, DREB2 is primarily associated with drought and salinity tolerance. In the chickpea genome, two genes, and , have been identified, exhibiting high sequence similarity to Arabidopsis genes. We investigated the nucleotide sequences of and genes in several chickpea cultivars commonly grown in northern Kazakhstan. Interestingly, the gene sequence was identical across all varieties and corresponded to the sequence deposited in the GenBank. However, the gene sequence exhibited variations among the studied varieties, categorized into three groups: the first group (I), comprising 20 cultivars, contained a gene sequence identical to the GenBank (Indian cultivar CDC Frontier). The second group (II), consisting of 4 cultivars, had a single synonymous substitution (T to C) compared to the deposited gene sequence. The third group, encompassing 5 cultivars, displayed one synonymous substitution (C to T) and two non-synonymous substitutions (G to T and G to A). Furthermore, we assessed the gene expression patterns of and in different chickpea varieties under drought conditions. Chickpea cultivars 8 (III), 37 (I), 6 (III), and 43 (I) exhibited the highest drought resistance. Our analysis revealed a strong positive correlation between drought resistance and gene expression under drought stress. Our findings suggest that the chickpea's adaptive responses to water deprivation are associated with changes in gene expression. To further elucidate the mechanisms underlying drought tolerance, we propose future research directions that will delve into the molecular interactions and downstream targets of genes.