Detection and Significance of Cell-Free DNA Mutation in Pleural Effusion in Patients with Advanced NSCLC

Objective. To detect EGFR/KRAS genes in pleural effusion cell-free DNA in patients with advanced non-small-cell lung cancer (NSCLC) and to explore the clinical significance of EGFR/KRAS mutation status in pleural effusion. Methods. A retrospective collection was performed on the specimens of pleural...

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Bibliographic Details
Published in:Emergency medicine international 2022-06, Vol.2022, p.3112281-5
Main Authors: Qiao, Man, Li, Dongsheng, He, Yuan, Zhang, Cen, Chi, Hang, Li, Xiaoqiu, Cui, QingMing, Li, ShaoYing, Jiao, Ying, Wei, Yuan
Format: Article
Language:English
Subjects:
Care and treatment
CEA (Oncology)
Codon
Comparative analysis
DNA
Gene mutations
Genetic aspects
Lung cancer, Non-small cell
Metastasis
Pleural effusions
Prognosis
Wei Yuan
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Summary:Objective. To detect EGFR/KRAS genes in pleural effusion cell-free DNA in patients with advanced non-small-cell lung cancer (NSCLC) and to explore the clinical significance of EGFR/KRAS mutation status in pleural effusion. Methods. A retrospective collection was performed on the specimens of pleural effusion and matched tissues from 50 patients with advanced NSCLC admitted to the hospital between January 2019 and January 2021. DNA mutation status of EGFR/KRAS in different specimens was detected and compared by pyrosequencing. The clinicopathological data and follow-up data of survival were collected. The relationship between DNA mutation and clinicopathological characteristics and prognosis was analyzed. Results. In the 50 pleural effusion specimens, there were 22 cases (44.00%) with EGFR mutations (19/21 exon mutations), including 12 cases with EGFR19 deletion mutation and 10 cases with EGFR21 exon L858R mutation. There were 6 cases (12.00%) with KRAS mutations (single-base substitution mutations), including 4 cases with 12-codon mutation and 2 cases with 13-codon mutation. In the 50 tissue specimens, there were 24 cases (48.00%) with EGFR mutations and 4 cases (8.00%) with KRAS mutations. There was no significant difference between pleural effusion specimens and tissue specimens, with good consistency (kappa = 0.920–0.779, P>0.05). EGFR mutation in pleural effusion was related to smoking history, types of pathological tissues, and lymph node metastasis (P
ISSN:2090-2840
2090-2859
DOI:10.1155/2022/3112281