The Potential Protective Role of RUNX1 in Nonalcoholic Fatty Liver Disease

• Published in: International journal of molecular sciences 2021-05, Vol.22 (10), p.5239
• Main Authors: Bertran, Laia, Pastor, Angela, Portillo-Carrasquer, Marta, Binetti, Jessica, Aguilar, Carmen, Martínez, Salomé, Vives, Margarita, Sabench, Fàtima, Porras, José Antonio, Riesco, David, Del Castillo, Daniel, Richart, Cristóbal, Auguet, Teresa
• Format: Article
• Language: English
• Subjects: Angiogenesis; Blood pressure; Cholesterol; Fatty acids; Fatty liver; Glucose; gut–liver axis; Histology; Immunohistochemistry; Inflammation; Insulin resistance; Jejunum; Lipid metabolism; Lipids; Liver; Liver diseases; Metabolism; mRNA; NAFLD; Obesity; Pathogenesis; Proteins; RUNX1; Runx1 protein; Small intestine; Steatosis; TLR4 protein; TLR5 protein; TLR9 protein; Toll-like receptors; Transcription factors; Tumor necrosis factor-TNF; Womens health
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22105239

The pathogenic mechanisms underlying nonalcoholic fatty liver disease (NAFLD) are beginning to be understood. RUNX1 is involved in angiogenesis, which is crucial in inflammation, but its role in nonalcoholic steatohepatitis (NASH) remains unclear. The aim of this study was to analyze RUNX1 mRNA hepatic and jejunal abundance in women with morbid obesity (MO) and NAFLD. RUNX1, lipid metabolism-related genes, and TLRs in women with MO and normal liver (NL, n = 28), NAFLD (n = 41) (simple steatosis (SS, n = 24), or NASH (n = 17)) were analyzed by RT-qPCR. The RUNX1 hepatic expression was higher in SS than in NL or NASH, as likewise confirmed by immunohistochemistry. An increased expression of hepatic FAS was found in NAFLD. Hepatic RUNX1 correlated positively with FAS. There were no significant differences in the jejunum RUNX1 expressions in the different groups. Jejunal FXR expression was lower in NASH than in NL, while the TLR9 expression increased as NAFLD progressed. Jejunal RUNX1 correlated positively with jejunal PPARγ, TLR4, and TLR5. In summary, the hepatic expression of RUNX1 seems to be involved in the first steps of the NAFLD process; however, in NASH, it seems to be downregulated. Our findings provide important insights into the role of RUNX1 in the context of NAFLD/NASH, suggesting a protective role.