The effect of laparoscopy on mast cell degranulation and mesothelium thickness in rats

Laparoscopy induces adhesion due to ischemia-reperfusion injury. However, the detail pathomechanism is poorly understood. This study aimed to investigate the impact of laparoscopy on mast cell and mesothelium morphological changes in the rat. Forty-nine males of Sprague-Dawley Rattus norvegicus were...

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Bibliographic Details
Published in:BMC surgery 2020-05, Vol.20 (1), p.111-111, Article 111
Main Authors: Poerwosusanta, Hery, Gunadi, Noor, Zairin, Oktaviyanti, Ika Kustiyah, Mintaroem, Karyono, Pardjianto, Bambang, Widodo, Moch Aris, Widjajanto, Edi
Format: Article
Language:English
Subjects:
Abdomen
Carbon dioxide
Catalase
Degranulation
Hydrogen peroxide
Infiltration
Intestine
Ischemia
Laparoscopic procedure
Laparoscopy
Liver
Malondialdehyde
mast cell infiltration and degranulation
Mesothelium
mesothelium thickness
Omentum
Oxidative stress
Peritoneum
Rats
Rattus norvegicus
Reperfusion
Small intestine
Superoxide dismutase
Superoxides
Surgery
Thickness
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Summary:Laparoscopy induces adhesion due to ischemia-reperfusion injury. However, the detail pathomechanism is poorly understood. This study aimed to investigate the impact of laparoscopy on mast cell and mesothelium morphological changes in the rat. Forty-nine males of Sprague-Dawley Rattus norvegicus were divided into four groups: a) control and b) intervention groups P1, P2, and P3 that underwent 60 min laparoscopic using carbon dioxide (CO ) insufflation at 8, 10, and 12 mmHg groups, respectively. Serum hydrogen peroxide (H O ), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and oxidative stress index (OSI) levels were determined 24 h after laparoscopy. Histopathological analyses of mast cell infiltration and degranulation and mesothelium thickness in the liver, greater omentum, mesenterium, small intestine, and peritoneum were performed 7 days after the procedure. H O , MDA, and OSI levels were significantly increased in the intervention groups compared with the control (p
ISSN:1471-2482
1471-2482
DOI:10.1186/s12893-020-00775-y