Study on the Relationship between Particulate Methane Monooxygenase and Methanobactin on Gold-Nanoparticles-Modified Electrodes

• Published in: Molecules (Basel, Switzerland) Switzerland), 2024-03, Vol.29 (6), p.1270
• Main Authors: Dou, Boxin, Li, Mingyu, Sun, Lirui, Xin, Jiaying, Xia, Chungu
• Format: Article
• Language: English
• Subjects: Air pollution; Catalytic oxidation; Copper - chemistry; Denaturation; direct electrochemistry; electrochemical; Electrodes; Electron transport; Electrons; Enzymes; Gold; Gold - chemistry; gold nanoparticles; Imidazoles; Kinetics; Metal Nanoparticles - chemistry; Methane; Methane - chemistry; methanobactin; Minerals; Nanoparticles; Oligopeptides; Oxidation-Reduction; Oxygenases; particulate methane monooxygenase; Peptides; Proteins
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29061270

(1) Background: Particulate methane monooxygenase (pMMO) has a strong dependence on the natural electron transfer path and is prone to denaturation, which results in its redox activity centers being unable to transfer electrons with bare electrodes directly and making it challenging to observe an electrochemical response; (2) Methods: Using methanobactin (Mb) as the electron transporter between gold electrodes and pMMO, a bionic interface with high biocompatibility and stability was created. The Mb-AuNPs-modified functionalized gold net electrode as a working electrode, the kinetic behaviors of pMMO bioelectrocatalysis, and the effect of Mb on pMMO were analyzed. The CV tests were performed at different scanning rates to obtain electrochemical kinetics parameters. (3) Results: The values of the electron transfer coefficient (α) and electron transfer rate constant ( ) are relatively large in test environments containing only CH or O . In contrast, in the test environment containing both CH and O , the bioelectrocatalysis of pMMO is a two-electron transfer process with a relatively small α and ; (4) Conclusions: It was inferred that Mb formed the complex with pMMO. More importantly, Mb not only played a role in electron transfer but also in stabilizing the enzyme structure of pMMO and maintaining a specific redox state. Furthermore, the continuous catalytic oxidation of natural substrate methane was realized.