Fly-FUCCI: A Versatile Tool for Studying Cell Proliferation in Complex Tissues

One promising approach for in vivo studies of cell proliferation is the FUCCI system (fluorescent ubiquitination-based cell cycle indicator). Here, we report the development of a Drosophila-specific FUCCI system (Fly-FUCCI) that allows one to distinguish G1, S, and G2 phases of interphase. Fly-FUCCI...

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Published in:Cell reports (Cambridge) 2014-04, Vol.7 (2), p.588-598
Main Authors: Zielke, Norman, Korzelius, Jerome, van Straaten, Monique, Bender, Katharina, Schuhknecht, Gregor F.P., Dutta, Devanjali, Xiang, Jinyi, Edgar, Bruce A.
Format: Article
Language:English
Subjects:
Anaphase-Promoting Complex-Cyclosome - genetics
Anaphase-Promoting Complex-Cyclosome - metabolism
Animals
Cell Cycle
Cell Line
Cell Proliferation
Cyclin B - genetics
Cyclin B - metabolism
Drosophila - cytology
Drosophila - genetics
Drosophila - metabolism
E2F1 Transcription Factor - genetics
E2F1 Transcription Factor - metabolism
Microscopy, Fluorescence - methods
Organ Specificity
Ubiquitination
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Summary:One promising approach for in vivo studies of cell proliferation is the FUCCI system (fluorescent ubiquitination-based cell cycle indicator). Here, we report the development of a Drosophila-specific FUCCI system (Fly-FUCCI) that allows one to distinguish G1, S, and G2 phases of interphase. Fly-FUCCI relies on fluorochrome-tagged degrons from the Cyclin B and E2F1 proteins, which are degraded by the ubiquitin E3-ligases APC/C and CRL4Cdt2, during mitosis or the onset of S phase, respectively. These probes can track cell-cycle patterns in cultured Drosophila cells, eye and wing imaginal discs, salivary glands, the adult midgut, and probably other tissues. To support a broad range of experimental applications, we have generated a toolkit of transgenic Drosophila lines that express the Fly-FUCCI probes under control of the UASt, UASp, QUAS, and ubiquitin promoters. The Fly-FUCCI system should be a valuable tool for visualizing cell-cycle activity during development, tissue homeostasis, and neoplastic growth. [Display omitted] •First functional FUCCI system for Drosophila•Tissue-specific expression of FUCCI probes•Toolbox of Fly-FUCCI reagents that includes transgenic flies, cell lines, and plasmids Edgar and colleagues report the development of the first functional FUCCI system for Drosophila, which allows accurate tracing of cell-cycle phases (G1, S, G2) and thus facilitates the detection of rare cell-cycle events in living tissues.
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2014.03.020