The Effect of White Rice and White Bread as Staple Foods on Gut Microbiota and Host Metabolism

• Published in: Nutrients 2018-09, Vol.10 (9), p.1323
• Main Authors: Mano, Fumika, Ikeda, Kaori, Joo, Erina, Fujita, Yoshihito, Yamane, Shunsuke, Harada, Norio, Inagaki, Nobuya
• Format: Article
• Language: English
• Subjects: Adult; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bread; Carbohydrates; Cross-Over Studies; Crossovers; Deoxyribonucleic acid; Diabetes; Diet; Dietary fiber; Dietary Fiber - administration & dosage; Dietary Fiber - metabolism; dietary pattern; DNA; Energy Metabolism; Fasting; Fatty acids; Feces; Feces - microbiology; Female; Gastrointestinal Microbiome; Gastrointestinal Tract - microbiology; Glucagon; Glucagon-like peptide 1; Grain; Host-Pathogen Interactions; Humans; intestinal biota; Intestinal microflora; Japan; Japanese diet; Laboratories; Laboratory testing; Male; Metabolism; Microbiota; Nutrition research; Nutritive Value; Oryza; Pilot Projects; Prebiotics; Rice; rice consumption; rRNA 16S; Thermal cycling
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu10091323

The purpose of this study was to examine the influence of two kinds of major Japanese staple foods, white rice and white bread, on gut microbiota against the background in which participants eat common side dishes. Seven healthy subjects completed the dietary intervention with two 1-week test periods with a 1-week wash-out period in cross-over design (UMIN registration UMIN000023142). White bread or white rice and 21 frozen prepared side dishes were consumed during the test periods. At baseline and at the end of each period, fasting blood samples, breath samples, and fecal samples were collected. For fecal samples, 16S rRNA gene sequencing was used to analyze the gut microbiota. After the bread period, the abundance of fecal genus (19.2 ± 14.5 vs. 6.2 ± 6.6 (%), = 0.03), fasting glucagon-like peptide 1 (GLP-1) (13.6 ± 2.0 vs. 10.5 ± 2.9 (pg/mL), = 0.03), and breath hydrogen (23.4 ± 9.9 vs. 8.2 ± 5.5 (ppm), = 0.02) were significantly higher than those of after the rice period. Plasma SCFAs also tended to be higher after the bread period. White bread contains more dietary fiber than refined short grain rice. These findings suggest that indigestible carbohydrate intake from short grain rice as a staple food may be smaller than that of white bread.